EasySep™ HLA Chimerism Whole Blood CD4 Positive Selection Kit
Immunomagnetic positive selection cell isolation kit
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EasySep™ HLA Chimerism Whole Blood CD4 Positive Selection Kit -
EasySep™ HLA Chimerism Whole Blood CD4 Positive Selection Kit -
Label for EasySep™ HLA Chimerism Whole Blood CD4 Positive Selection Kit
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Required Products
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Cell separation buffer -
"The Big Easy" EasySep™ Magnet
Magnet for column-free immunomagnetic separation
Overview
The EasySep™ HLA Chimerism Whole Blood CD4 Positive Selection is designed to isolate CD4+ cells from fresh whole blood or buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD4 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD4 antigen is expressed on helper T cells and more weakly on monocytes and tissue macrophages.
This product replaces the EasySep™ Human Whole Blood CD4 Positive Selection Kit (Catalog #18082) for even faster cell isolations.
This product replaces the EasySep™ Human Whole Blood CD4 Positive Selection Kit (Catalog #18082) for even faster cell isolations.
Advantages:
• Fast and easy-to-use
• Up to 99% purity
• No columns required
• Up to 99% purity
• No columns required
Components:
- EasySep™ HLA Chimerism Whole Blood CD4 Positive Selection Kit (Catalog #17888)
- EasySep™ HLA Chimerism Whole Blood CD4 Positive Selection Cocktail, 3 x 1 mL
- EasySep™ Dextran RapidSpheres™ (Catalog #50100), 3 x 1 mL
- EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
- RoboSep™ HLA Chimerism Whole Blood CD4 Positive Selection Kit with Filter Tips (Catalog #17888RF)
- EasySep™ HLA Chimerism Whole Blood CD4 Positive Selection Cocktail, 3 x 1 mL
- EasySep™ Dextran RapidSpheres™ (Catalog #50100), 3 x 1 mL
- EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
- RoboSep™ Buffer (Catalog #20104) x 2
- RoboSep™ Filter Tips (Catalog #20125) x 2
Magnet Compatibility:
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep Magnet™ (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype:
Cell Isolation Kits
Cell Type:
T Cells, CD4+
Species:
Human
Sample Source:
Buffy Coat; Peripheral Blood
Selection Method:
Positive
Application:
Cell Isolation
Brand:
EasySep; RoboSep
Area of Interest:
Chimerism; HLA; Immunology
Related Products
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EasySep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit
Immunomagnetic positive selection cell isolation kit -
EasySep™ HLA Chimerism Whole Blood CD8 Positive Selection Kit
Immunomagnetic positive selection cell isolation kit -
EasySep™ HLA Chimerism Whole Blood CD3 Positive Selection Kit
Immunomagnetic positive selection cell isolation kit -
EasySep™ HLA Chimerism Whole Blood CD19 Positive Selection Kit
Immunomagnetic positive selection cell isolation kit
Scientific Resources
Product Documentation
Document Type
Product Name
Catalog #
Lot #
Language
17888
All
17888
All
17888RF
All
17888RF
All
17888RF
All
17888RF
All
17888RF
All
Educational Materials
(12)
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages for
Workflow Stages
Data and Publications
Data
Starting with human whole blood, the CD4+CD14- cell content of the isolated fraction is typically 97.0 ± 1.8% (gated on CD45; mean ± SD using “The Big Easy” EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 29.8% and 99.0%, respectively. NOTE: RBCs were removed from the start sample by ammonium chloride lysis prior to flow cytometry.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.