EasySep™ HLA Chimerism Whole Blood CD19 Positive Selection Kit

Immunomagnetic positive selection cell isolation kit
Catalog #
17874_C
Immunomagnetic positive selection cell isolation kit
From: 659 USD
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Required Products
  1. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

  2. "The Big Easy" EasySep™ Magnet
    "The Big Easy" EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

Overview

The EasySep™ HLA Chimerism Whole Blood CD19 Positive Selection Kit is designed to isolate CD19+ cells from whole blood or buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD19 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD19 antigen is present on mature B cells and B cell progenitors but is lost upon maturation to plasma cells. It is also expressed on follicular dendritic cells.

This product replaces the EasySep™ Human Whole Blood CD19 Positive Selection Kit (Catalog #18084) for even faster cell isolations.
Advantages
• Fast and easy-to-use
• Up to 99% purity
• No columns required
• Compatible across EasySep™ "The Big Easy", EasyEights™, and RoboSep™-S platforms
Components
  • EasySep™ HLA Chimerism Whole Blood CD19 Positive Selection Kit (Catalog #17874)
    • EasySep™ HLA Chimerism Whole Blood CD19 Positive Selection Cocktail, 3 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50101, 3 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
  • RoboSep™ HLA Chimerism Whole Blood CD19 Positive Selection Kit (Catalog #17874RF)
    • EasySep™ HLA Chimerism Whole Blood CD19 Positive Selection Cocktail, 3 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50101, 3 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
    • RoboSep™ Buffer (Catalog #20104) x 2
    • RoboSep™ Filter Tips (Catalog #20125) x 2
Magnet Compatibility
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
B Cells
Species
Human
Sample Source
Buffy Coat, Whole Blood
Selection Method
Positive
Brand
EasySep
Area of Interest
HLA, Immunology

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet - Buffy Coat or Whole Blood
Product Name
EasySep™ HLA Chimerism Whole Blood CD19 Positive Selection Kit
Catalog #
17874
Lot #
All
Language
English
Document Type
Product Information Sheet - Leukoreduction System Chamber/Cone
Product Name
EasySep™ HLA Chimerism Whole Blood CD19 Positive Selection Kit
Catalog #
17874
Lot #
All
Language
English
Document Type
Product Information Sheet - Buffy Coat or Whole Blood
Product Name
RoboSep™ HLA Chimerism Whole Blood CD19 Positive Selection Kit
Catalog #
17874RF
Lot #
All
Language
English
Document Type
Product Information Sheet - Leukoreduction System Chamber/Cone
Product Name
RoboSep™ HLA Chimerism Whole Blood CD19 Positive Selection Kit
Catalog #
17874RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ HLA Chimerism Whole Blood CD19 Positive Selection Kit
Catalog #
17874
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ HLA Chimerism Whole Blood CD19 Positive Selection Kit
Catalog #
17874
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ HLA Chimerism Whole Blood CD19 Positive Selection Kit
Catalog #
17874RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ HLA Chimerism Whole Blood CD19 Positive Selection Kit
Catalog #
17874RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ HLA Chimerism Whole Blood CD19 Positive Selection Kit
Catalog #
17874RF
Lot #
All
Language
English

Educational Materials(14)

Brochure
EasySep™ Cell Separation Technology
Brochure
Human B Cell Isolation Product Selection
Brochure
HLA Antibodies for Purity Assessment
Brochure
Cell Separation Products for HLA Analysis
Technical Bulletin
Fully Automated, High Purity Cell Isolation for Chimerism Labs
Technical Bulletin
Purity Assessment by Flow Cytometry for Lineage-Specific Chimerism Analysis
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Wallchart
Human Immune Cytokines
Video
4:55
How to Recover Cells from a Leukocyte Reduction System (LRS) Cone
Video
1:41
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument
Video
1:57
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Webinar
56:02
Fully Automated Cell Separation for Chimerism Analysis with RoboSep™
Webinar
59:59
Increasing the Sensitivity of Cytogenetic Analysis of Hematologic Malignancies Through Cell Enrichment
Scientific Poster
Isolation of Very Pure Lymphoid (CD3+, CD19+ or CD56+) and Myeloid (CD15+ or CD33/CD66b+) Cell Subsets in as Little as 15 Minutes for Use in Lineage-Specific Chimerism Analysis
Load More Educational Materials

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Starting with human whole blood, the CD19+ cell content of the isolated fraction typically ranges from 94.3 - 99.6% (as assessed by staining the start and isolated fractions with anti-CD19 or anti-CD20 antibodies, respectively). In the above example, the purities of the start and the final isolated fractions are 5.0% and 98.6%, respectively (gated on CD45).
NOTE: Red blood cells were removed from the start sample by lysis prior to flow cytometry.

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