EasySep™ Direct Human B Cell Isolation Kit
Overview
This cell isolation kit targets non-B cells for removal with EasySep™ Direct RapidSpheres™ and antibodies recognizing specific surface antigens. Unwanted cells are separated from desired cells using an EasySep™ magnet, and untouched target cells are then pipetted or poured into a new tube.
Isolated cells can be used directly in downstream applications such as flow cytometry, culture or DNA/RNA extraction.
• Up to 95% purity of isolated cells
• Fast, easy-to-use and column-free
• Isolated cells are untouched
- EasySep™ Direct Human B Cell Isolation Kit (Catalog #19674)
- EasySep™ Direct Human B Cell Isolation Cocktail, 2 x 2.5 mL
- EasySep™ Direct RapidSpheres™, 4 x 2.5 mL
- RoboSep™ Human B Cell Isolation Kit (Catalog #19674RF)
- EasySep™ Direct Human B Cell Isolation Cocktail, 2 x 2.5 mL
- EasySep™ Direct RapidSpheres™, 4 x 2.5 mL
- RoboSep™ Buffer (Catalog #20104)
- RoboSep™ Filter Tips (Catalog #20125) x 2
Scientific Resources
Product Documentation
Document Type | Product Name | Catalog # | Lot # | Language |
---|---|---|---|---|
Document Type
Product Information Sheet
|
Product Name
EasySep™ Direct Human B Cell Isolation Kit
|
Catalog #
19674 |
Lot #
All |
Language
English |
Document Type
Product Information Sheet
|
Product Name
EasySep™ Direct Human B Cell Isolation Kit for RoboSep™
|
Catalog #
19674RF |
Lot #
All |
Language
English |
Document Type
Safety Data Sheet 1
|
Product Name
EasySep™ Direct Human B Cell Isolation Kit
|
Catalog #
19674 |
Lot #
All |
Language
English |
Document Type
Safety Data Sheet 2
|
Product Name
EasySep™ Direct Human B Cell Isolation Kit
|
Catalog #
19674 |
Lot #
All |
Language
English |
Document Type
Safety Data Sheet 1
|
Product Name
EasySep™ Direct Human B Cell Isolation Kit for RoboSep™
|
Catalog #
19674RF |
Lot #
All |
Language
English |
Document Type
Safety Data Sheet 2
|
Product Name
EasySep™ Direct Human B Cell Isolation Kit for RoboSep™
|
Catalog #
19674RF |
Lot #
All |
Language
English |
Educational Materials (15)
Frequently Asked Question
Can EasySep™ be used for either positive or negative selection?
How does the separation work?
Which columns do I use?
How can I analyze the purity of my enriched sample?
Can EasySep™ separations be automated?
Can EasySep™ be used to isolate rare cells?
Are the EasySep™ magnetic particles FACS-compatible?
Can the EasySep™ magnetic particles be removed after enrichment?
Can I alter the separation time in the magnet?
For positive selection, can I perform more than 3 separations to increase purity?
How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?
If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
Data

Figure 1. Typical EasySep™ Direct Human B Cell Isolation Profile
Starting with human whole blood from normal healthy donors, the typical B cell (CD3-CD19+) content of the non-lysed final isolated fraction is 95.3 ± 2.7% (gated on CD45) or 88.5 ± 11.5% (not gated on CD45). In the above example, the B cell (CD3-CD19+) content of the lysed whole blood start sample and the non-lysed final isolated fraction is 6.2% and 95.9% (gated on CD45), respectively, or 6.2% and 95.8% (not gated on CD45), respectively. The starting frequency of B cells in the non-lysed whole blood start sample above is 0.011% (data not shown).
Publications (2)
Abstract
Abstract
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.