Anti-Phospho-SHIP Antibody, Polyclonal

Rabbit polyclonal antibody against human, mouse phosphoSHIP

Anti-Phospho-SHIP Antibody, Polyclonal

Rabbit polyclonal antibody against human, mouse phosphoSHIP

From: 581 USD
Catalog #
60142_C
Rabbit polyclonal antibody against human, mouse phosphoSHIP

Overview

Phospho-SHIP is a highly phosphorylated form of SHIP. SH2-containing inositol phosphatase (SHIP) is a 145 kDa hematopoietic-restricted protein that becomes tyrosine-phosphorylated and associated with the adaptor protein, Shc, following cytokine, growth factor, chemokine, and immunoreceptor stimulation. SHIP also hydrolyzes the critical phosphatidylinositol (PI)-3-kinase (PI3K)-generated second messenger, PI-3,4,5-P3 (PIP3), to PI-3,4-P2 1,2 and therefore acts as an important negative regulator of the PI3K pathway. This antibody reacts with mouse and human tyrosine-phosphorylated SHIP.
Subtype
Primary Antibodies
Target Antigen
PhosphoSHIP
Alternative Names
p150Ship; phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1; SHIP-1; SHIP1; SIP-145; s-SHIP
Reactive Species
Human, Mouse
Conjugation
Unconjugated
Host Species
Rabbit
Cell Type
Granulocytes and Subsets, Lymphocytes, Myeloid Cells
Species
Human, Mouse
Application
Flow Cytometry, Immunoblotting, Immunofluorescence, Immunoprecipitation
Area of Interest
Immunology
Clone
Not applicable
Gene ID
3635 (human), 16331 (mouse)
Isotype
Not applicable

Scientific Resources

Product Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
60142
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60142
Lot #
All
Language
English

Data and Publications

(A) Flow cytometry analysis of bone marrow derived mast cells (BMMCs) from normal (+/+) or SHIP knockout (-/-) mice. Cells were stimulated with stem cell factor (SCF; filled histogram) or left unstimulated (solid line historgram), and then fixed, permeabilized, and labeled with Anti-Phospho-SHIP Antibody, Polyclonal, followed by an anti-rabbit Alexa Fluor® 488.

(B) BMMCs were either stimulated with SCF or left unstimulated, and then fixed, permeabilized, and labeled with Anti-Phospho-SHIP Antibody, Polyclonal, followed by an anti-rabbit Alexa Fluor® 488.

(C) Western blot analysis of total cell lysates from human TF-1 cells stimulated with IL-3, mouse WEHI-231 B cells stimulated with anti-IgM, and mouse BMMCs stimulated with SCF, and probed with Anti-Phospho-SHIP Antibody, Polyclonal. The blot was re-probed with an anti-SHIP antibody to show equal loading of unstimulated (C) and stimulated (S) samples. Phospho-SHIP has a predicted molecular mass of 145 kDa.

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