Anti-Mouse CD19 Antibody, Clone 6D5
Rat monoclonal IgG2a antibody against mouse CD19
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Overview
This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse B Cell Isolation Kit (Catalog #19854).
Data Figures
Figure 1. Data for Alexa Fluor® 488-Conjugated
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 6D5, Alexa Fluor® 488 (filled histogram) or a rat IgG2a, kappa Alexa Fluor® 488 isotype control antibody (open histogram).
Figure 2. Data for PE-Conjugated
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 6D5, PE (filled histogram) or a rat IgG2a, kappa PE isotype control antibody (open histogram).
Figure 3. Data for Unconjugated
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 6D5, followed by a mouse anti-rat IgG2a antibody, FITC (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758 (Catalog #60076) followed by a mouse anti-rat IgG2a antibody, FITC (solid line histogram).
Figure 4. Data for APC-Conjugated
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 6D5, APC (filled histogram) or a rat IgG2a, kappa APC isotype control antibody (solid line histogram).
Figure 5. Data for Biotin-Conjugated
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 6D5, Biotin followed by streptavidin (SAV) APC (filled histogram) or a rat IgG2a, kappa biotin isotype control antibody followed by SAV APC (solid line histogram).
Figure 6. Data for FITC-Conjugated
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 6D5, FITC (filled histogram) or a rat IgG2a, kappa FITC isotype control antibody (solid line histogram).
Figure 7. Data for PB-Conjugated
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 6D5, Pacific Blue™ (filled histogram) or a rat IgG2a, kappa Pacific Blue™ isotype control antibody (solid line histogram).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (4)
Related Products
Legal Statement:
Alexa Fluor and Pacific Blue are trademarks of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® or Pacific Blue™ are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
