Anti-Mouse CD19 Antibody, Clone 6D5

Rat monoclonal IgG2a antibody against mouse CD19

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Rat monoclonal IgG2a antibody against mouse CD19
From: 104 USD

Overview Related Products Scientific Resources Product Applications Data and Publications

Overview

The 6D5 antibody reacts with CD19, an ~95 kDa type 1 transmembrane glycoprotein expressed on the surface of B cells throughout all stages of development, from early pre-B cells to plasma cells. Expression is down-regulated but persists in terminally differentiated plasma cells. CD19 is also found on follicular dendritic cells. By associating with CD21 and CD81, CD19 functions as a co-receptor for the B cell receptor and is involved in B cell activation and differentiation. Activation of CD19 is accompanied by phosphorylation of the cytoplasmic domain, which promotes binding to kinases and the induction of intracellular signaling cascades. Mutations in CD19 can result in severe immunodeficiency syndromes. Clone 6D5 recognizes the same epitope as clone ID3 in cross-competition assays.

This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse B Cell Isolation Kit (Catalog #19854).
Subtype:
Primary Antibodies
Target Antigen:
CD19
Alternative Names:
B4
Reactive Species:
Mouse
Conjugation:
Alexa Fluor 488; APC; Biotin; FITC; PE; Unconjugated; Pacific Blue
Host Species:
Rat
Cell Type:
B Cells
Application:
Cell Isolation; Flow Cytometry; CyTOF; Immunocytochemistry; Immunofluorescence; Immunohistochemistry; Immunoprecipitation; Western Blotting
Area of Interest:
Immunology
Clone:
6D5
Gene ID:
12478
Isotype:
IgG2a, kappa

Scientific Resources

Product Documentation

Educational Materials

(4)
Brochure
Hematopoietic Stem and Progenitor Cells - Products for Your Research
Brochure
Reliable Antibodies for Your Research
Brochure
Tools For Your Immunology Research
Wallchart
Human Immune Cytokines

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area Workflow Stages for
Workflow Stages
B Cell Research
Cell Sourcing
Cell Isolation
Cell Expansion
Cell Characterization
Dendritic Cell Research
Cell Sourcing
Cell Isolation
Differentiation and Maturation
Cell Characterization
Hematopoietic Stem and Progenitor Cell Research
Cell Sourcing and Isolation
Expansion and Differentiation
Analysis
Total Lymphocyte Research
Cell Sourcing
Cell Isolation
Cell Characterization

Data and Publications

Data

Data for Alexa Fluor® 488-Conjugated

Figure 1. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 6D5, Alexa Fluor® 488 (filled histogram) or a rat IgG2a, kappa Alexa Fluor® 488 isotype control antibody (open histogram).

Data for PE-Conjugated

Figure 2. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 6D5, PE (filled histogram) or a rat IgG2a, kappa PE isotype control antibody (open histogram).

Data for Unconjugated

Figure 3. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 6D5, followed by a mouse anti-rat IgG2a antibody, FITC (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758 (Catalog #60076) followed by a mouse anti-rat IgG2a antibody, FITC (solid line histogram).

Data for APC-Conjugated

Figure 4. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 6D5, APC (filled histogram) or a rat IgG2a, kappa APC isotype control antibody (solid line histogram).

Data for Biotin-Conjugated

Figure 5. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 6D5, Biotin followed by streptavidin (SAV) APC (filled histogram) or a rat IgG2a, kappa biotin isotype control antibody followed by SAV APC (solid line histogram).

Data for FITC-Conjugated

Figure 6. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 6D5, FITC (filled histogram) or a rat IgG2a, kappa FITC isotype control antibody (solid line histogram).

Figure 7. Data for PB-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 6D5, Pacific Blue™ (filled histogram) or a rat IgG2a, kappa Pacific Blue™ isotype control antibody (solid line histogram).

Alexa Fluor and Pacific Blue are trademarks of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® or Pacific Blue™ are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.