Overview
This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit (Catalog #19856).
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(2)Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
Data

Figure 1. Data for Alexa Fluor® 488-Conjugated
(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD117 Antibody, Clone 2B8, Alexa Fluor® 488 and anti-mouse CD45R APC. (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells pre-labeled with Anti-Mouse CD117 Antibody, Clone 2B8, Alexa Fluor® 488 and processed with the EasySep™ Mouse CD117 Positive Selection Kit (Catalog #18757). Histograms show labeling of bone marrow (Start) and isolated cells (Isolated); partial blocking may be observed. Labeling with a rat IgG2b, kappa Alexa Fluor® 488 isotype control antibody is shown (solid line histogram).

Figure 2. Data for PE-Conjugated
(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD117 Antibody, Clone 2B8, PE and anti-mouse CD45R APC. (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells processed with the EasySep™ Mouse PE Positive Selection Kit (Catalog #18554), and labeled with Anti-Mouse CD117 Antibody, Clone 2B8, PE. Histograms show labeling of bone marrow (Start) and isolated cells (Isolated). Labeling with a rat IgG2b, kappa PE isotype control antibody is shown (solid line histogram).

Figure 3. Data for Unconjugated
(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD117 (c-Kit) Antibody, Clone 2B8, followed by a mouse anti-rat IgG2b antibody, FITC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE (Catalog #60019PE). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530 (Catalog #60077), followed by a mouse anti-rat IgG2b antibody, FITC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE.

Figure 4. Data for APC-Conjugated
(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD117 Antibody, Clone 2B8, APC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, FITC (Catalog #60019FI). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, APC (Catalog #60077AZ) and Anti-Mouse CD45R Antibody, Clone RA3-6B2, FITC.

Figure 5. Data for Biotin-Conjugated
(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD117 Antibody, Clone 2B8, Biotin, followed by streptavidin (SAV) APC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, FITC (Catalog #60019FI). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, Biotin (Catalog #60077BT), followed by SAV APC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, FITC.

Figure 6. Data for FITC-Conjugated
(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD117 Antibody, Clone 2B8, FITC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, APC (Catalog #60019AZ). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, FITC (Catalog #60077FI) and Anti-Mouse CD45R Antibody, Clone RA3-6B2, APC.