Anti-Human CD33 Antibody, Clone HIM3-4

Mouse monoclonal IgG1 antibody against human, chimpanzee CD33

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Mouse monoclonal IgG1 antibody against human, chimpanzee CD33
From: 108 USD

Overview Related Products Scientific Resources Product Applications Data and Publications

Overview

The HIM3-4 antibody reacts with CD33 (Siglec-3), an ~67 - 75 kDa type 1 transmembrane sialoadhesion protein of the immunoglobulin superfamily. CD33 is expressed on committed myeloid progenitor cells, monocytes, granulocytes, dendritic cells, mast cells, resident macrophages, and subsets of B cells and activated T and NK cells. CD33 is a major marker used to differentiate myeloid from lymphoid or erythroid leukemias. In peripheral blood it is expressed at high levels on monocytes and at lower levels on granulocytes. CD33 functions as a sialic acid-dependent cell adhesion molecule and mediates protein-glycan and protein-protein interactions between cells. It also has a putative role as an inhibitory receptor during the immune response. HIM3-4 reportedly binds to the extracellular C2 Ig domain of CD33, and does not block subsequent binding of several other anti-CD33 clones, including P67.6, WM53, and WM54. Two isoforms designated CD33M and CD33m have been identified and among these clones only HIM3-4 recognizes both isoforms.
Subtype:
Primary Antibodies
Target Antigen:
CD33
Alternative Names:
gp67, p67, Sialic acid-binding immunoglobulin-like lectin 3, SIGLEC3, Siglec-3
Reactive Species:
Chimpanzee; Human
Conjugation:
FITC; PE; Unconjugated
Host Species:
Mouse
Cell Type:
Granulocytes and Subsets; Myeloid Cells
Application:
Flow Cytometry; Immunocytochemistry; Immunofluorescence
Area of Interest:
Immunology
Clone:
HIM3-4
Gene ID:
945
Isotype:
IgG1, kappa

Scientific Resources

Product Documentation

Educational Materials

(3)
Brochure
Reliable Antibodies for Your Research
Brochure
Tools For Your Immunology Research
Wallchart
Human Immune Cytokines

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area Workflow Stages for
Workflow Stages
Myeloid Cell Research
Cell Sourcing
Cell Isolation
Cell Characterization
Chimerism Analysis
Cell Isolation
Characterization

Data and Publications

Data

Data for FITC-Conjugated

Figure 1. Data for FITC-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD33 Antibody, Clone HIM3-4, FITC (filled histogram) or a mouse IgG1, kappa FITC isotype control antibody (black line histogram).
(B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD14 Positive Selection Kit and labeled with Anti-Human CD33 Antibody, Clone HIM3-4, FITC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse Ig1, kappa FITC isotype control antibody is shown (open histogram).

Data for Unconjugated

Figure 2. Data for Unconjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD33 Antibody, Clone HIM3-4, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or a mouse IgG1, kappa isotype control antibody, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).

Data for PE-Conjugated

Figure 3. Data for PE-Conjugated

Flow cytometry analysis of human buffy coat nucleated cells labeled with Anti-Human CD33 Antibody, Clone HIM3-4, PE (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PE (Catalog #60070PE) (solid line histogram).

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.