Anti-Human CD235a (Glycophorin A) Antibody, Clone 2B7

Mouse monoclonal IgG1 antibody against human CD235a (glycophorin A)

Anti-Human CD235a (Glycophorin A) Antibody, Clone 2B7

Mouse monoclonal IgG1 antibody against human CD235a (glycophorin A)

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Mouse monoclonal IgG1 antibody against human CD235a (glycophorin A)
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Overview

The 2B7 antibody reacts with CD235a (Glycophorin A), an ~10 kDa type I sialoglycoprotein present in the cell membrane of erythrocytes and erythroid precursors as a homodimer. Glycophorin A bears the antigenic determinants for the MN and Ss blood groups and has been proposed to provide a large mucin-like surface to erythrocytes that acts to minimize aggregation in circulation. Glycophorin A is first detectable on morphologically recognizable erythroid precursors just after the colony-forming unit erythroid (CFU-E) stage, and reaches its maximal expression at the late normoblast stage. Anti-glycophorin is useful in combination with anti-transferrin receptor (CD71) to identify distinct stages of erythroid differentiation since CD71 expression precedes Glycophorin A expression, but is lost during maturation of normoblasts into mature red blood cells (RBCs). Peptide-binding ELISA data indicate that the epitope of the 2B7 antibody is located in the extracellular domain of CD235a within the sequence Ala54 - Ser66 (AATPRAHEVSEIS).
Subtype
Primary Antibodies
Target Antigen
CD235 (Glycophorin)
Reactive Species
Human
Conjugation
FITC
Host Species
Mouse
Cell Type
Erythroid Cells, Red Blood Cells
Species
Human
Application
Cell Isolation, Flow Cytometry
Area of Interest
Immunology

Data Figures

Flow cytometry analysis of human whole blood labeled with Anti-Human CD235a (Glycophorin A) Antibody, Clone 2B7, FITC (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, FITC (Catalog #60070FI; solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
60152FI
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60152FI
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications