EasySep™ Human Th17 Cell Enrichment Kit II

Immunomagnetic cell isolation kit using particle release technology

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EasySep™ Human Th17 Cell Enrichment Kit II

Immunomagnetic cell isolation kit using particle release technology

From: 2,302 USD
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Immunomagnetic cell isolation kit using particle release technology
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Product Advantages


  • Fast and easy-to-use

  • Up to 98% purity

  • No columns required

What's Included

  • EasySep™ Human Th17 Enrichment Kit II (Catalog #17862)
    • EasySep™ Human CCR6 Positive Selection Cocktail II, 0.5 mL
    • EasySep™ Releasable RapidSpheres™, 4 x 1 mL
    • EasySep™ Human CD4+CXCR3- T Cell Pre-Enrichment Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™, 1 mL
    • EasySep™ Release Buffer, 2 x 1 mL

Overview

The EasySep™ Human Th17 Enrichment Kit II is a two-step method designed to enrich for human Th17 cells from fresh peripheral blood mononuclear cells. First, CCR6+ cells are isolated by column-free immunomagnetic positive selection using antibody complexes and EasySep™ Releasable RapidSpheres™. Then, bound magnetic particles are removed from the cells and unwanted non-CD4+ T cells, CD45RA+ cells, and CXCR3+ cells are targeted for depletion using antibody complexes and EasySep™ Dextran RapidSpheres™. The final isolated fraction is enriched for IL-17-producing Th17 cells.

This product can be used in place of the EasySep™ Human Th17 Cell Enrichment Kit (Catalog #18162) for even faster cell isolations.
Subtype
Cell Isolation Kits
Cell Type
T Cells, CD4+
Species
Human
Sample Source
Leukapheresis, PBMC
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep
Area of Interest
Immunology

Data Figures


Starting with fresh PBMCs, the Th17 cell content (CD4+CXCR3-CCR6+) of the isolated fraction typically ranges from 96 - 98%. Following overnight stimulation with PMA-Ionomycin, 6 - 19% of the isolated cells are IL-17+ by intracellular flow cytometry. These values vary widely among donors. IFN-γ-producing cells are typically < 5% of the enriched fraction.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

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17862
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English
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Safety Data Sheet 1
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17862
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English
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Safety Data Sheet 2
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17862
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English
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Safety Data Sheet 3
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English
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Safety Data Sheet 4
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English
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Safety Data Sheet 5
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17862
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Safety Data Sheet 6
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17862
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All
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English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more