Human Recombinant IL-8 (CXCL8)

Interleukin 8
Catalog #
78084_C
Interleukin 8
From: 185 USD

Overview

Interleukin-8 (IL-8) is a member of the CXC subfamily of chemokines and is produced by leukocytic cells (monocytes, T cells, neutrophils, and natural killer cells) and non-leukocytic somatic cells (endothelial cells, fibroblasts, and epithelial cells), with the most prominent source being monocytes and macrophages. Its production is induced by inflammatory stimuli, such as IL-1. IL-8, also known as CXCL8, activates neutrophils inducing chemotaxis, exocytosis, and the respiratory burst (Baggiolini & Clark-Lewis; Mukaida). IL-8 is considered one of the most potent neutrophil chemoattractants in inflammation and binds to two different chemokine receptors on leukocytes: the G protein-coupled receptors CXCR1 and CXCR2 (Hoffmann et al.; de Oliveira et al.). IL-8 has angiogenic effects on human intestinal microvascular endothelial cells in vitro that are mediated by CXCR2 (Heidemann et al.). IL-8 is reported to promote breast cancer progression by increasing cell invasion, angiogenesis, and metastasis and has been reported to be involved in regulating breast cancer stem-like cells (Singh et al.). IL-8 also has proangiogenic properties in inflammatory diseases of the conjunctiva, cornea, iris, retina, and orbit (Ghasemi et al.). It was also shown that a major T cell effector function in human newborns is IL-8 production, which has the potential to activate antimicrobial neutrophils and gamma/delta T cells (Gibbons et al.). A variety of human pathogens, such as HIV and Mycobacterium tuberculosis, have been shown to induce IL-8 production by monocytes and macrophages (Friedland et al.; Meddows-Taylor et al.).
Subtype
Cytokines
Alternative Names
CXC motif ligand 8, GCP-1, Granulocyte chemotactic protein 1, Interleukin-8, MDNCF, Monocyte-derived neutrophil chemotactic factor, NAF, NAP-1, Neutrophil activating factor, SCYB8, Small inducible cytokine subfamily B member 8
Cell Type
Airway Cells, Endothelial Cells, Keratinocytes, Macrophages, Monocytes, NK Cells
Species
Human
Area of Interest
Epithelial Cell Biology, Immunology, Stem Cell Biology
Molecular Weight
8.9 kDa
Purity
≥ 95 %

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Human Recombinant IL-8 (CXCL8)
Catalog #
78084.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Human Recombinant IL-8 (CXCL8)
Catalog #
78084.1
Lot #
All
Language
English

Educational Materials (3)

Brochure
Tools For Your Immunology Research
Brochure
Cytokines, Chemokines and Growth Factors
Wallchart
Human Immune Cytokines

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

(A) The biological activity of Human Recombinant IL-8 (CXCL8) was tested by its ability to mobilize Ca2+ in CHO-K1/Gα15/hCXCR1 cells (human Gα15 and human CXCR1 stably expressed in CHO-K1 cells). Ca2+ mobilization was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which Ca2+ mobilization is at 50% of maximum. The EC50 in the example above is less than 0.15 μg/mL. (B) 2 μg of Human Recombinant IL-8 (CXCL8) was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant IL-8 (CXCL8) has a predicted molecular mass of 8.9 kDa.

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