StemSpan™ Myeloid Expansion Supplement (100X)

Serum-free culture supplement for expansion and differentiation of human granulocytes

StemSpan™ Myeloid Expansion Supplement (100X)

Serum-free culture supplement for expansion and differentiation of human granulocytes

StemSpan™ Myeloid Expansion Supplement (100X)
1 mL
724 USD
Catalog # 02693

Serum-free culture supplement for expansion and differentiation of human granulocytes

Product Advantages

• Promotes the production of thousands of CD15+ granulocytes per input CD34+ human CB cell in 14-day liquid cultures.
• Supplied as a 100X concentrate. After thawing and mixing, the tube contents can be added directly to any hematopoietic cell expansion medium of choice.

Overview

StemSpan™ Myeloid Expansion Supplement (100X) contains a combination of recombinant human cytokines formulated to selectively promote the expansion and differentiation of human myeloid progenitor cells, generating large numbers of CD15+ granulocytes from CD34+ cells isolated from human cord blood (CB) or bone marrow (BM) samples.

StemSpan™ Myeloid Expansion Supplement is intended for use in combination with any of the following StemSpan™ media:
• StemSpan™ SFEM (Catalog #09600)
• StemSpan™ SFEM II (Catalog #09605)
• StemSpan™-XF (Catalog #100-0073)
• StemSpan™-AOF (Catalog #100-0130)

When added to serum-free medium, StemSpan™ Myeloid Expansion Supplement typically promotes the production of thousands of CD15+ granulocytes per input CD34+ cell in 14-day liquid cultures initiated with CD34+ human CB cells. See data tab for more details.
Contains
• Recombinant human stem cell factor (SCF)
• Recombinant human thrombopoietin (TPO)
• Recombinant human granulocyte colony-stimulating factor (G-CSF)
• Recombinant human granulocyte/macrophage colony-stimulating factor (GM-CSF)
Subtype
Supplements
Cell Type
Hematopoietic Stem and Progenitor Cells, Myeloid Cells
Species
Human
Application
Cell Culture, Differentiation, Expansion
Brand
StemSpan
Area of Interest
Stem Cell Biology, Transplantation Research
Formulation
Serum-Free

Scientific Resources

Product Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
02693
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
02693
Lot #
All
Language
English

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Production of CD13+ and CD15+ Myeloid Cells by the Expansion and Lineage-Specific Differentiation of Human BM-Derived Cells Cultured in StemSpan™ SFEM II Containing Myeloid CD34+ Expansion Supplement

Figure 1. Production of CD13+ and CD15+ Myeloid Cells by the Expansion and Lineage-Specific Differentiation of Human BM-Derived Cells Cultured in StemSpan™ SFEM II Containing Myeloid Expansion Supplement

Flow cytometry dot plots for 14-day cultures of human BM-derived CD34+ cells in StemSpan™ SFEM II containing Myeloid Expansion Supplement showing expression of the HSPC marker CD34, and pan-hematopoietic marker CD45 (A) before and (B) after 14 days of culture, and expression of myeloid markers CD13 and CD15 (C) after 14 days of culture. The frequency of CD34+ cells declined from 83% before culture to ~1% after 14 days. In parallel, myeloid CD13+CD15+ cells gradually accumulated from <10% on day 0 to 46% by day 14. The frequency of CD14+ cells after culture was low (typically <10%, see Table 1).

Table 1. Production of Myeloid Cells from Human CB-Derived CD34+ Cells Cultured in StemSpan™ SFEM II Containing Myeloid Expansion Supplement

Production of Myeloid Cells from Human CB-Derived CD34+ Cells Cultured in StemSpan™ SFEM II Containing Myeloid Expansion Supplement

Number of total nucleated cells (TNCs) produced per input human CB-derived CD34+ cell from 14 different donors and percentage of cells positive for myeloid markers CD13, CD14 and CD15 produced after 14 days of culture in SFEM II containing Myeloid Expansion Supplement. Best expansion was obtained in SFEM II, but myeloid cell expansion may also be obtained in SFEM and ACF media.
*95% confidence limits (CL), the range within which 95% of the results will typically fall.

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