Rat Recombinant TNF-alpha

Tumor necrosis factor alpha
Catalog #
78124_C
Tumor necrosis factor alpha
From: 342 USD

Overview

Tumor necrosis factor-alpha (TNF-α) is a pro-inflammatory cytokine that activates NF-κB, MAPK, and PI3K/AKT pathways. Activated T cells and macrophages are the primary producers of TNF-α in response to inflammation and infectious conditions. Many other cell types have been shown to produce TNF-α, among them B cells, NK cells, mast cells, neutrophils, dendritic cells, microglia, endothelial cells, smooth muscle cells, cardiomyocytes, and fibroblasts. TNF-α has cytotoxic effects on cancerous cells by stimulating anti-tumor immunosuppressive responses. TNF-α stimulates expression of E- and P-selectins, thus facilitating adhesion of neutrophils, monocytes, and memory T cells to activated platelets and endothelial cells (Zelová & Hošek). Other effects of TNF-α include vasodilatation and edema formation. In vitro studies of adult rat neural progenitor cells (NPCs) demonstrate that TNF-α reduces neurogenesis in dentate gyrus-derived NPCs, and promotes astrogliogenesis in subventricular zone-derived NPCs (Borsini et al.).
Subtype
Cytokines
Alternative Names
Cachectin, Cytotoxin, Differentiation-inducing factor, DIF, Necrosin, TNFA, TNFSF2, Tumor necrosis factor
Cell Type
Granulocytes and Subsets, Monocytes, T Cells
Species
Rat
Area of Interest
Immunology
Molecular Weight
17.4 kDa
Purity
≥ 95%

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Rat Recombinant TNF-alpha
Catalog #
78124.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Rat Recombinant TNF-alpha
Catalog #
78124.1
Lot #
All
Language
English

Educational Materials (1)

Wallchart
Human Immune Cytokines

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

(A) The biological activity of Rat Recombinant TNF-alpha was tested by its ability to inhibit cell growth of mouse L929 cells. Cell viability was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is less than 0.05 ng/mL. (B) 2 μg of Rat Recombinant TNF-alpha was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.

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