Human Peripheral Blood B Cells, Frozen

Primary human cells, frozen

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Human Peripheral Blood B Cells, Frozen

Primary human cells, frozen

From: 1,174 USD

Size

2 x 10^7 cells 1 x 10^7 cells

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Primary human cells, frozen

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Overview

Human primary B cells derived from peripheral blood (PB) are isolated from mononuclear cells (MNCs) using the negative immunomagnetic selection technique. Cells are untouched and ready for downstream applications. PB was collected using acid-citrate-dextrose solution A (ACDA) as the anticoagulant.

Cells were obtained using Institutional Review Board (IRB)-approved consent forms and protocols.

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Browse our Frequently Asked Questions (FAQs) on Primary Cells.

• CryoStor® CS10

Frozen

B Cells

Human

Peripheral Blood

Normal

≥ 85% CD19+ by flow cytometry

Data Figures

Figure 1. Cryopreserved B Cells Show High Viability and Produce IgG Antibodies Upon Stimulation

(A) B Cells (Catalog #70023) cryopreserved in CryoStor® CS10 (Catalog #07930) show high viability upon thawing (average = 96.3 ± 0.6 %, n = 6). (B) B cells were cultured for 1 week in RPMI 1640 Medium (Catalog #36750) with 10% FBS, 2mM L-Glutamine (Catalog #07100), 10mM HEPES (Catalog #07200) and 55 µM β-mercaptoethanol and either left unstimulated (control) or stimulated with CD40 in the presence of IL-21 (activated). Activated B cells produce significantly more IgG antibodies compared to unstimulated controls as measured by ELISA.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type

Product Name

Catalog #

Lot #

Language

Document Type

Product Information Sheet

Product Name

Human Peripheral Blood B Cells, Frozen

Catalog #

70023, 70023.1

Lot #

All

Language

English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area

Workflow Stages

Workflow Stages for B Cell Research

Cell Sourcing

Cell Isolation

Cell Expansion

Cell Characterization

Workflow Stages for Total Lymphocyte Research

Cell Sourcing

Cell Isolation

Cell Characterization

Resources and Publications

Related Products

Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

Human Peripheral Blood B Cells, Frozen

Primary human cells, frozen

Human Peripheral Blood B Cells, Frozen

Primary human cells, frozen

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Overview

Data Figures

Protocols and Documentation

Applications

Resources and Publications

Educational Materials (4)

Publications (1)

Abstract

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