Extracellular Vesicle Research

Traditional density gradient-based methods for EV isolation such as ultracentrifugation often require access to specialized equipment, involve time-consuming protocols and do not result in high purities or quantities of EVs. In contrast, EasySep™ Human Extracellular Vesicle Positive Selection Kits use immunomagnetic separation to isolate highly purified human EVs from biological samples quickly—in as little as 30 minutes—for downstream characterization by Western blot, mass spectrometry, and qPCR. See MoreThis easy-to-use method targets EVs for positive selection with antibodies recognizing EV and EV subset markers, and generates a higher yield compared to ultracentrifugation.

Size exclusion chromatography (SEC) also provides an easy and efficient method for separation of EVs from circulating proteins1 and can be performed rapidly and cost-effectively using Extracellular Vesicle SEC Columns. SEC causes minimal alteration in vesicle characteristics such as functionality and shape2. Also, as the isolated EVs are untouched, with no particles, antibodies, or polymers attached to them during and following isolation, they are suitable for use in downstream cell culture applications.

  1. Baranyai T et al. (2015) Isolation of exosomes from blood plasma: qualitative and quantitative comparison of ultracentrifugation and size exclusion chromatography methods. PLoS One 10(12): e0145686.
  2. Gámez-Valero A et al. (2016) Size-exclusion chromatography-based isolation minimally alters extracellular vesicles’ characteristics compared to precipitating agents. Sci Rep 6(1): 33641.
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Cell Isolation Products 4 (5)

Laboratory Equipment 4 (2)

Cultureware and General Supplies 4 (4)

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