Extracellular Vesicle SEC Columns
Size exclusion chromatography (SEC) column for isolation of extracellular vesicles from biological fluids
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In-lab demonstration
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Costar® Microcentrifuge TubesNon-sterile, clear, polypropylene snap-cap microcentifuge tubes
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D-PBS (Without Ca++ and Mg++)Dulbecco’s phosphate-buffered saline without calcium and magnesium
Overview
The EV isolation protocol using the SEC columns is easy and takes approximately 15 minutes. The columns are available in three sizes for different sample volume ranges and can be used up to five times.
Data Figures
Figure 1. Isolation of Extracellular Vesicles from Plasma Using a 0.5 mL Extracellular Vesicle SEC Column
The 0.5 mL Extracellular Vesicle SEC Column was loaded with 0.5 mL of human plasma. 100 μL fractions were collected and analyzed for particle and protein content by nanoparticle tracking analysis (NTA) and bicinchoninic acid (BCA) assays, respectively. EVs were detected in fractions 9 - 14, while proteins were detected in fractions 15 onwards.
Figure 2. Isolation of Extracellular Vesicles from Plasma Using a 2 mL Extracellular Vesicle SEC Column
The 2 mL Extracellular Vesicle SEC Column was loaded with 2 mL of human plasma. 500 μL fractions were collected and analyzed for particle and protein content by nanoparticle tracking analysis and bicinchoninic acid assays, respectively. EVs were detected in fractions 7 - 10, while proteins were detected in fractions 11 onwards.
Figure 3. Isolation of Extracellular Vesicles from Conditioned Medium Using a 20 mL Extracellular Vesicle SEC Column
The 20 mL Extracellular Vesicle SEC Column was loaded with 20 mL of 10-fold concentrated serum-free medium conditioned by mesenchymal stromal cell (MSC) culture. 1 mL fractions were collected and analyzed for particle and protein content by nanoparticle tracking analysis and bicinchoninic acid assays, respectively. EVs were detected in fractions 15 - 30, while proteins were detected in fractions 35 onwards. Fractions 31-33, which are outside of the typical EV elution range, may still contain EVs with low protein contamination for serum-free media samples.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (3)
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Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
