Module 2: Preparing Cells for EasySep™ - Frequently Used Processing Methods for Human Blood Samples
Learning Objectives
In this module, you will learn:
- Which common anticoagulants are used for blood sample collection
- How to isolate PBMCs, cryopreserve them, and thaw them for isolation
- How to prepare a buffy coat
- How to remove red blood cells (RBCs)
- How to remove platelets
This session will take approximately 25 minutes to complete.
Supplementary Resources
- Slides Module 2 - Frequently Used Processing Methods for Human Blood Samples
- Video How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
- Resource SepMate™ PBMC Isolation
- Resource Human PBMC Isolation, Freezing, Thawing, Sourcing, and More
- Protocol How to Remove Granulocytes from Old Blood Samples
- Video How to Quickly Thaw Frozen Cells with the ThawStar® CFT2 Automated Thawing System
- Protocol How to Reduce Cell Clumping in Single-Cell Suspensions Using DNase I
- Protocol How to Prepare a Buffy Coat from Whole Blood
- Video How to Isolate Nucleated Cells from Whole Blood Using HetaSep™ Erythrocyte Aggregation Agent
- Brochure EasySep™ RBC Depletion Reagent
- Tech Tip Reducing Platelet Contamination in Blood Samples
