On-Demand Course: Human Immune Cell Isolation

Welcome to the on-demand course on EasySep™ Human Immune Cell Isolation. In this self-paced, virtual course, you will learn how to prepare human blood samples, perform cell separation with EasySep™, and analyze the performance of your isolations. Relevant technical resources on cell processing will also be shared in the course. Prior to starting, we encourage you to review the course curriculum. When you’re ready, begin with the first module.

Before You Begin

The training curriculum consists of five modules, some with associated videos illustrating the laboratory procedures discussed in the modules. The modules should be reviewed sequentially. The modules range from approximately 15 - 25 minutes in duration and the entire course can be completed in approximately 2 hours. For any questions or additional support through each stage of the protocol, please contact the Product and Scientific Support team at techsupport@stemcell.com.


Instructor Bio

Dr. Lu Lu

Dr. Lu Lu, Specialist, Immunology, Scientific Support

Lu is an Immunology Specialist in the Scientific Support Department at STEMCELL Technologies. Lu obtained her PhD in Virology from the University of Hong Kong with a focus on using adjuvants to improve vaccine effectiveness. Prior to joining STEMCELL, she was an Assistant Research Officer and led COVID-19-related team projects. At STEMCELL, Lu provides frontline support with our product portfolios to the global customer base and sales staff, supporting research, biotech, and diagnostic labs. She specializes in STEMCELL’s immunology product portfolio, including cell separation and immune cell culture products, and is also a trainer in corporate training programs with a focus on immunology products and protocols.


Course Curriculum

  • Common human peripheral blood samples compatible with EasySep™
  • How to use human blood samples with EasySep™
  • How to process leukopaks (LP) for EasySep™
  • How to process Leukocyte Reduction System Cones (LRSC) for EasySep™

Mini Quiz: Test Your Knowledge


Estimated time for completion: 20 minutes

  • Common anticoagulants used for blood sample collection
  • How to isolate PBMCs, cryopreserve them, and thaw them for isolation
  • How to prepare a buffy coat
  • How to remove red blood cells (RBCs)
  • How to remove platelets

Mini Quiz: Test Your Knowledge


Estimated time for completion: 25 minutes

  • What EasySep™ cell separation technology is
  • What EasySep™ strategies are available
  • What cells can be isolated with EasySep™
  • How to choose an EasySep™ product for your isolation

Mini Quiz: Test Your Knowledge


Estimated time for completion: 20 minutes

  • How to execute the key steps when performing cell separation with EasySep™, including:
    • Before cell separation with EasySep™: Storing the kit components, preparing the starting sample, and selecting a magnet
    • During cell separation with EasySep™: Adding reagents and buffer, removing the supernatant after magnetic separation, and resuspending the particle-bound cells

Mini Quiz: Test Your Knowledge


Estimated time for completion: 20 minutes

  • How to assess the separation performance after EasySep™ by purity and recovery analysis

Mini Quiz: Test Your Knowledge


Estimated time for completion: 20 minutes

Complete this final assessment to test your knowledge and receive a certificate of completion.


Estimated time for completion: 20 minutes