Mouse IgM ELISA Antibody Pair Kit
Overview
The assay is based on the sandwich ELISA method, in which samples are added to ELISA plates coated with capture antibodies specific for the immunoglobulin. The captured immunoglobulin is detected by addition of a biotinylated detection antibody, followed by streptavidin-alkaline phosphatase (SA-ALP), which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate p-nitrophenyl phosphate (pNPP) results in a colored product with an intensity directly proportional to the concentration of immunoglobulin in the sample. The concentration of the immunoglobulin is determined by comparison to a serial dilution of the immunoglobulin standard analyzed in parallel.
NOTE: This kit includes sufficient reagents for 6 x 96-well ELISA plates (Catalog #38019). ELISA plates and pNPP Substrate (Catalog #01917) are required for use with the Mouse IgM ELISA Antibody Pair Kit and are available for purchase separately.
- Mouse IgM Capture Antibody, 300 µL
- Mouse IgM Biotinylated Detection Antibody, 80 µL
- SA-ALP, 80 µL
- Mouse IgM Standard, 1 vial
- Standard Reconstitution Buffer B, 1 mL
Scientific Resources
Product Documentation
Document Type | Product Name | Catalog # | Lot # | Language |
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Document Type
Product Information Sheet
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Product Name
Mouse IgM ELISA Antibody Pair Kit
|
Catalog #
01999 |
Lot #
All |
Language
English |
Document Type
Safety Data Sheet 1
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Product Name
Mouse IgM ELISA Antibody Pair Kit
|
Catalog #
01999 |
Lot #
All |
Language
English |
Document Type
Safety Data Sheet 2
|
Product Name
Mouse IgM ELISA Antibody Pair Kit
|
Catalog #
01999 |
Lot #
All |
Language
English |
Document Type
Safety Data Sheet 3
|
Product Name
Mouse IgM ELISA Antibody Pair Kit
|
Catalog #
01999 |
Lot #
All |
Language
English |
Document Type
Safety Data Sheet 4
|
Product Name
Mouse IgM ELISA Antibody Pair Kit
|
Catalog #
01999 |
Lot #
All |
Language
English |
Document Type
Safety Data Sheet 5
|
Product Name
Mouse IgM ELISA Antibody Pair Kit
|
Catalog #
01999 |
Lot #
All |
Language
English |
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
Data

Representative Standard Curve
• Reportable Range: 0.1 - 25 ng/mL. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity.
• Accuracy: No international standard exists for calibration.
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.