How to Isolate Mononuclear Cells from Whole Blood by Density Gradient Centrifugation

This step-by-step technical guide demonstrates how to isolate peripheral blood mononuclear cells from whole blood using density gradient centrifugation (e.g. Ficoll-Paque™, Lymphoprep™). See how to layer blood over the density medium and learn two different ways of harvesting cells from the blood plasma:density medium interface.

Publish Date: March 13, 2012 Views: 122775