Growth of Organoids in PancreaCult™

Follow the growth of pancreatic organoids yourself. Pancreatic organoid fragments at passage 6 were seeded into Matrigel™ domes, cultured in PancreaCult™ and then imaged in an IncuCyte Zoom (Essen BioScience) every three hours over a period of 7 days. Organoids are seen growing in size until collapsing on themselves. Dead cells are visualised with propidium iodide (red). The optimal period for organoid passaging in this culture is seen to occur between days 3 and 6, when the majority of organoids have reached their maximum growth, and have not collapsed.
Publish Date: April 18, 2018 Views: 0