EasySep™ Human Th17 Cell Enrichment Kit II

Immunomagnetic cell isolation kit using particle release technology

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EasySep™ Human Th17 Cell Enrichment Kit II

Immunomagnetic positive selection of human Th17 cells

2 x 109 cells
Catalog #17862
1,807 USD

Required Products

Overview Related Products Scientific Resources Product Applications Data and Publications

Overview

The EasySep™ Human Th17 Enrichment Kit II is a two-step method designed to enrich for human Th17 cells from fresh peripheral blood mononuclear cells. First, CCR6+ cells are isolated by column-free immunomagnetic positive selection using antibody complexes and EasySep™ Releasable RapidSpheres™. Then, bound magnetic particles are removed from the cells and unwanted non-CD4+ T cells, CD45RA+ cells, and CXCR3+ cells are targeted for depletion using antibody complexes and EasySep™ Dextran RapidSpheres™. The final isolated fraction is enriched for IL-17-producing Th17 cells.

This product can be used in place of the EasySep™ Human Th17 Cell Enrichment Kit (Catalog #18162) for even faster cell isolations.
Advantages:
• Fast and easy-to-use
• Up to 98% purity
• No columns required
Components:
• EasySep™ Human Th17 Enrichment Kit II (Catalog #17862)
     • EasySep™ Human CCR6 Positive Selection Cocktail II, 0.5 mL
     • EasySep™ Releasable RapidSpheres™, 4 x 1 mL
     • EasySep™ Human CD4+CXCR3- T Cell Pre-Enrichment Cocktail, 1 mL
     • EasySep™ Dextran RapidSpheres™, 1 mL
     • EasySep™ Release Buffer, 2 x 1 mL
Subtype:
Cell Isolation Kits
Cell Type:
T Cells, CD4+; T Cells
Species:
Human
Sample Source:
Leukapheresis; PBMC
Selection Method:
Positive
Application:
Cell Isolation
Brand:
EasySep
Area of Interest:
Immunology

Scientific Resources

Product Documentation

Educational Materials

(7)
Brochure
Tools For Your Immunology Research
Brochure
EasySep™ Cell Separation Technology
Wallchart
Human Immune Cytokines
Video
1:37
How to Isolate Mononuclear Cells from Whole Blood by Density Gradient Centrifugation
Video
1:57
Fast and Easy Cell Isolation with EasySep™
Video
1:13
The EasySep™ Pour-off: A Simple and Effective Way to Isolate Cells
Video
1:23
How to Isolate Cells with EasySep™

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area Workflow Stages for
Workflow Stages
T Cell Research
Cell Sourcing
Cell Isolation
Cell Activation and Expansion
Cell Characterization
T Cell Engineering
Cell Isolation
Cell Activation and Expansion
Cell Characterization
Cell Cryopreservation

Data and Publications

Data


Starting with fresh PBMCs, the Th17 cell content (CD4+CXCR3-CCR6+) of the isolated fraction typically ranges from 96 - 98%. Following overnight stimulation with PMA-Ionomycin, 6 - 19% of the isolated cells are IL-17+ by intracellular flow cytometry. These values vary widely among donors. IFN-γ-producing cells are typically < 5% of the enriched fraction.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.