EasySep™ Human Th17 Cell Enrichment Kit II
Immunomagnetic cell isolation kit using particle release technology
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EasySep™ Human Th17 Cell Enrichment Kit II -
Label for EasySep™ Human Th17 Cell Enrichment Kit II
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Required Products
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Magnet for column-free immunomagnetic separation -
Cell separation buffer -
Compatible antibodies for purity assessment of isolated cells
Overview
The EasySep™ Human Th17 Enrichment Kit II is a two-step method designed to enrich for human Th17 cells from fresh peripheral blood mononuclear cells. First, CCR6+ cells are isolated by column-free immunomagnetic positive selection using antibody complexes and EasySep™ Releasable RapidSpheres™. Then, bound magnetic particles are removed from the cells and unwanted non-CD4+ T cells, CD45RA+ cells, and CXCR3+ cells are targeted for depletion using antibody complexes and EasySep™ Dextran RapidSpheres™. The final isolated fraction is enriched for IL-17-producing Th17 cells.
This product can be used in place of the EasySep™ Human Th17 Cell Enrichment Kit (Catalog #18162) for even faster cell isolations.
This product can be used in place of the EasySep™ Human Th17 Cell Enrichment Kit (Catalog #18162) for even faster cell isolations.
Advantages:
• Fast and easy-to-use
• Up to 98% purity
• No columns required
• Up to 98% purity
• No columns required
Components:
- EasySep™ Human Th17 Enrichment Kit II (Catalog #17862)
- EasySep™ Human CCR6 Positive Selection Cocktail II, 0.5 mL
- EasySep™ Releasable RapidSpheres™, 4 x 1 mL
- EasySep™ Human CD4+CXCR3- T Cell Pre-Enrichment Cocktail, 1 mL
- EasySep™ Dextran RapidSpheres™, 1 mL
- EasySep™ Release Buffer, 2 x 1 mL
Subtype:
Cell Isolation Kits
Cell Type:
T Cells; T Cells, CD4+
Species:
Human
Sample Source:
Leukapheresis; PBMC
Selection Method:
Positive
Application:
Cell Isolation
Brand:
EasySep
Area of Interest:
Immunology
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Human Peripheral Blood Leukopak, Fresh
Primary human cells, fresh
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Anti-Human CD4 Antibody, Clone OKT4
Mouse monoclonal IgG2b antibody against human, rhesus, cynomolgus CD4
Scientific Resources
Product Documentation
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Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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Data and Publications
Data
Starting with fresh PBMCs, the Th17 cell content (CD4+CXCR3-CCR6+) of the isolated fraction typically ranges from 96 - 98%. Following overnight stimulation with PMA-Ionomycin, 6 - 19% of the isolated cells are IL-17+ by intracellular flow cytometry. These values vary widely among donors. IFN-γ-producing cells are typically < 5% of the enriched fraction.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.