Anti-Mouse CD19 Antibody, Clone 6D5

Rat monoclonal IgG2a antibody against mouse CD19
Catalog #
60006_C
Rat monoclonal IgG2a antibody against mouse CD19
From: 104 USD

Overview

The 6D5 antibody reacts with CD19, an ~95 kDa type 1 transmembrane glycoprotein expressed on the surface of B cells throughout all stages of development, from early pre-B cells to plasma cells. Expression is down-regulated but persists in terminally differentiated plasma cells. CD19 is also found on follicular dendritic cells. By associating with CD21 and CD81, CD19 functions as a co-receptor for the B cell receptor and is involved in B cell activation and differentiation. Activation of CD19 is accompanied by phosphorylation of the cytoplasmic domain, which promotes binding to kinases and the induction of intracellular signaling cascades. Mutations in CD19 can result in severe immunodeficiency syndromes. Clone 6D5 recognizes the same epitope as clone ID3 in cross-competition assays.

This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse B Cell Isolation Kit (Catalog #19854).
Subtype
Primary Antibodies
Target Antigen
CD19
Alternative Names
B4
Reactive Species
Mouse
Conjugation
Alexa Fluor 488, Pacific Blue, APC, Biotin, FITC, PE, Unconjugated
Host Species
Rat
Cell Type
B Cells
Species
Mouse
Application
Cell Isolation, CyTOF, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
Area of Interest
Immunology
Clone
6D5
Gene ID
12478
Isotype
IgG2a, kappa

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD19 Antibody, Clone 6D5
Catalog #
60006
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD19 Antibody, Clone 6D5, Alexa Fluor® 488
Catalog #
60006AD, 60006AD.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD19 Antibody, Clone 6D5, APC
Catalog #
60006AZ, 60006AZ.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD19 Antibody, Clone 6D5, Biotin
Catalog #
60006BT, 60006BT.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD19 Antibody, Clone 6D5, FITC
Catalog #
60006FI, 60006FI.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD19 Antibody, Clone 6D5, PE
Catalog #
60006PE, 60006PE.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD19 Antibody, Clone 6D5, Pacific Blue™
Catalog #
60006PB, 60006PB.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD19 Antibody, Clone 6D5
Catalog #
60006
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD19 Antibody, Clone 6D5, Alexa Fluor® 488
Catalog #
60006AD, 60006AD.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD19 Antibody, Clone 6D5, APC
Catalog #
60006AZ, 60006AZ.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD19 Antibody, Clone 6D5, Biotin
Catalog #
60006BT, 60006BT.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD19 Antibody, Clone 6D5, FITC
Catalog #
60006FI, 60006FI.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD19 Antibody, Clone 6D5, PE
Catalog #
60006PE, 60006PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD19 Antibody, Clone 6D5, Pacific Blue™
Catalog #
60006PB, 60006PB.1
Lot #
All
Language
English

Educational Materials (4)

Brochure
Tools For Your Immunology Research
Brochure
Hematopoietic Stem and Progenitor Cells - Products for Your Research
Brochure
Reliable Antibodies for Your Research
Wallchart
Human Immune Cytokines

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Data for Alexa Fluor® 488-Conjugated

Figure 1. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 6D5, Alexa Fluor® 488 (filled histogram) or a rat IgG2a, kappa Alexa Fluor® 488 isotype control antibody (open histogram).

Data for PE-Conjugated

Figure 2. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 6D5, PE (filled histogram) or a rat IgG2a, kappa PE isotype control antibody (open histogram).

Data for Unconjugated

Figure 3. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 6D5, followed by a mouse anti-rat IgG2a antibody, FITC (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758 (Catalog #60076) followed by a mouse anti-rat IgG2a antibody, FITC (solid line histogram).

Data for APC-Conjugated

Figure 4. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 6D5, APC (filled histogram) or a rat IgG2a, kappa APC isotype control antibody (solid line histogram).

Data for Biotin-Conjugated

Figure 5. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 6D5, Biotin followed by streptavidin (SAV) APC (filled histogram) or a rat IgG2a, kappa biotin isotype control antibody followed by SAV APC (solid line histogram).

Data for FITC-Conjugated

Figure 6. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 6D5, FITC (filled histogram) or a rat IgG2a, kappa FITC isotype control antibody (solid line histogram).

Figure 7. Data for PB-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 6D5, Pacific Blue™ (filled histogram) or a rat IgG2a, kappa Pacific Blue™ isotype control antibody (solid line histogram).

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