Anti-Human MUC1 (CD227) Antibody, Clone 214D4

Mouse monoclonal IgG1 antibody against human MUC1 (CD227)

Anti-Human MUC1 (CD227) Antibody, Clone 214D4

Mouse monoclonal IgG1 antibody against human MUC1 (CD227)

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Mouse monoclonal IgG1 antibody against human MUC1 (CD227)
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Overview

The 214D4 antibody reacts with MUC1 (also known as CD227), a large cell surface glycoprotein expressed by most glandular and ductal epithelial cells as well as a variety of hematopoietic cells. A characteristic feature of the MUC1 (CD227) glycoprotein is a core protein domain composed of a variable number of tandem repeats and multiple oligosaccharide side chains. Because the extracellular portion of MUC1 can extend beyond most cell surface proteins, it is thought to play a role in cell-cell and cell-substrate adhesion. The protein is highly expressed by the majority of human adenocarcinomas and is associated with a poor prognosis. In the mammary gland, MUC1 is localized on the apical plasma membrane of luminal epithelial cells. The 214D4 antibody clone is particularly useful since it enriches for luminal-restricted but not bipotent epithelial progenitor cells from normal human mammary tissue.
Subtype
Primary Antibodies
Target Antigen
MUC1 (CD227)
Alternative Names
CD227, EMA, Episialin, Epithelial membrane antigen, HMFG antigen, MAM6, Mucin 1, PEM, Polymorphic epithelial mucin
Reactive Species
Human
Conjugation
Unconjugated
Host Species
Mouse
Cell Type
Mammary Cells
Species
Human
Application
Cell Isolation, Immunohistochemistry
Area of Interest
Epithelial Cell Biology, Stem Cell Biology
Clone
214D4
Gene ID
4582
Isotype
IgG1

Data Figures

Flow cytometry analysis of human MCF7 cells labeled with Anti-Human MUC1 (CD227) Antibody, Clone 214D4, followed by a rat anti-mouse IgG1 antibody, FITC (filled histogram) or a mouse IgG1, kappa isotype control antibody, followed by a rat anti-mouse IgG1 antibody, FITC (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
60137
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60137
Lot #
All
Language
English

Applications

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Resources and Publications

Educational Materials (1)