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Protocol Resources for Cryopreservation of Cell Cultures

Preparation and Preservation

Cryopreservation

Cryopreservation or cell freezing is the process of using low temperatures to preserve cells and tissues for future use. Depending on the cell type of interest, there can be a variety of protocols and methods to choose from. Here you will find useful protocols for freezing or thawing different cell types, including human pluripotent stem cells, organoids, and primary cells.

To learn about the fundamentals of cryopreservation, view our Cryopreservation Basics Guide >

Protocol How to Cryopreserve Intestinal Organoids
Protocol Cryopreserving Hepatic Organoids Expanded in HepatiCult™ Organoid Growth Medium (Human)
Protocol How to Thaw Intestinal Organoids
Tech Tip Refrigerate, Freeze, or Fix Cells for Flow Cytometry or Storage
Protocol How to Cryopreserve Human Pluripotent Stem Cells in CryoStor® CS10
Protocol How to Cryopreserve Neurospheres
Tech Tip Cryopreservation and Thawing of Pluripotent Stem Cells: Technical Tips and Media Recommendations
Protocol Cryopreserving Human Liver Tissue for Organoid Culture
Protocol How to Thaw Frozen Primary Cells
Tech Tip Storing Cord Blood Samples
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Product Information Sheets

For detailed protocols developed specifically for our products, please consult the Product Information Sheet or enter the product keyword or catalog number below.


Try CryoStor® CS10, a serum-free freezing medium, for stable and long-term cell storage.
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