How to Model the Human Airway at the Air-Liquid Interface: Introduction
Protocols for expanding and differentiating human bronchial epithelial cells (HBECs) at the air-liquid interface (ALI) using the PneumaCult™ culture system to demonstrate ALI culture
In this series of videos, we will guide you through the protocols for expanding and differentiating human bronchial epithelial cells (HBECs) at the air-liquid interface (ALI). We will use the PneumaCult™ culture system to demonstrate ALI culture for this video. If you are using other culture media, some alterations to this protocol may be necessary.
00:48 Overview of the ALI culture workflow using PneumaCult™
01:02 Overview of phase 1- Expansion culture in a flask
01:15 Overview of phase 2 - Submerged expansion in porous inserts
01:37 Overview of phase 3 - ALI culture differentiation in inserts
Watch the other videos in this series
Video 1: How to Model the Human Airway at the ALI: Expansion of HBECs
Video 2: How to Model the Human Airway at the Air-Liquid Interface: Differentiation of HBECs
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How to Model the Human Airway at the Air-Liquid Interface: Expansion of HBECs
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Serum- and BPE-free medium for human airway epithelial cells cultured at the air-liquid interface
