How to Model the Human Airway at the Air-Liquid Interface: Expansion of HBECs
Learn how to expand human bronchial epithelial cells (HBECs) to initiate an air-liquid interface (ALI) culture using PneumaCult™ culture media
In this protocol video, you will learn how to expand human bronchial epithelial cells (HBECs) to initiate an air-liquid interface (ALI) culture using PneumaCult™ culture media.
00:35 Phase 1: Expansion Culture in Flasks
01:37 Plating
02:45 Morphological Assessment
05:36 Phase 2: Submerged Expansion in Inserts
Watch the other videos in this series:
How to Model the Human Airway at the Air-Liquid Interface: Introduction
How to Model the Human Airway at the Air-Liquid Interface: Differentiation of HBECs
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How to Model the Human Airway at the Air-Liquid Interface: Introduction
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