Media Additives for hPSC Workflows
The TeSR™ family of feeder-free maintenance media is optimized to support and maintain high-quality human pluripotent stem cell (hPSCs) cultures, including embryonic stem (ES) and induced pluripotent stem (iPS) cells. In general, our TeSR™ media is a complete media, consisting of basal and supplement components, tailored for different needs. It does not require other media additives during routine cell aggregate passaging as outlined in the respective TeSR™ media protocol. Our guidance is to keep the media simple. Hence, it is not necessary to add antibiotics to our TeSR™ media as it may place a selective pressure for a cell line adapted to grow well only when those antibiotics are present in the medium and suppress the detection of culture contamination.
However, there are situations where hPSCs are subjected to stressful conditions in which media additives are required and recommended to improve cell survival and assay performance. STEMCELL Technologies offers several cell culture supplements designed for specific cell applications, such as hPSC single-cell cloning workflows, single-cell sorting, thawing, passaging sensitive cell lines, and plating single cells. This Technical Tip provides a quick comparison chart highlighting several supplements and their recommended applications to help you select the right one for your work.
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