Resveratrol

Antioxidant; COX-1 inhibitor

Resveratrol

Antioxidant; COX-1 inhibitor

From: 125 USD
Catalog #
72862_C
Antioxidant; COX-1 inhibitor

Overview

Resveratrol is a potent phenolic antioxidant found in grapes and red wine that also has antiproliferative and anti-inflammatory activity (Rotondo et al.). Resveratrol is also a selective inhibitor of cyclooxygenase-1 (COX-1; Jang) with an ED₅₀ value of 15 μM, respectively.

MAINTENANCE AND SELF-RENEWAL
· Inhibits differentiation of pig preadipocytes into adipocytes (Bai et al.).
· Promotes self-renewal of human mesenchymal stem cells (Peltz et al.).

DIFFERENTIATION
· Induces osteogenic differentiation in human bone marrow-derived mesenchymal stem cell cultures (Boissy et al.; Dai et al.; Peltz et al.).
· Induces osteoblast differentiation while blocking adipocyte development in mouse mesenchymal stem cells and cultured primary rat bone marrow cells (Bäckesjö et al.).
Alternative Names
(E)-Resveratrol; trans-Resveratrol
Cell Type
Mesenchymal Stem and Progenitor Cells, Osteoblasts
Species
Human, Mouse, Rat, Non-Human Primate, Other
Application
Differentiation, Maintenance
Area of Interest
Stem Cell Biology
CAS Number
501-36-0
Chemical Formula
C₁₄H₁₂O₃
Molecular Weight
228.2 g/mol
Purity
≥ 98%
Target
COX-1

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Product Name
Resveratrol
Catalog #
72862, 72864
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Resveratrol
Catalog #
72862, 72864
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications

Educational Materials (2)

Publications (7)

Resveratrol exerts dosage and duration dependent effect on human mesenchymal stem cell development. Peltz L et al. PloS one 2012 JAN

Abstract

Studies in the past have illuminated the potential benefit of resveratrol as an anticancer (pro-apoptosis) and life-extending (pro-survival) compound. However, these two different effects were observed at different concentration ranges. Studies of resveratrol in a wide range of concentrations on the same cell type are lacking, which is necessary to comprehend its diverse and sometimes contradictory cellular effects. In this study, we examined the effects of resveratrol on cell self-renewal and differentiation of human mesenchymal stem cells (hMSCs), a type of adult stem cells that reside in a number of tissues, at concentrations ranging from 0.1 to 10 µM after both short- and long-term exposure. Our results reveal that at 0.1 µM, resveratrol promotes cell self-renewal by inhibiting cellular senescence, whereas at 5 µM or above, resveratrol inhibits cell self-renewal by increasing senescence rate, cell doubling time and S-phase cell cycle arrest. At 1 µM, its effect on cell self-renewal is minimal but after long-term exposure it exerts an inhibitory effect, accompanied with increased senescence rate. At all concentrations, resveratrol promotes osteogenic differentiation in a dosage dependent manner, which is offset by its inhibitory effect on cell self-renewal at high concentrations. On the contrary, resveratrol suppresses adipogenic differentiation during short-term exposure but promotes this process after long-term exposure. Our study implicates that resveratrol is the most beneficial to stem cell development at 0.1 µM and caution should be taken in applying resveratrol as an anticancer therapeutic agent or nutraceutical supplement due to its dosage dependent effect on hMSCs.
Activation of Sirt1 decreases adipocyte formation during osteoblast differentiation of mesenchymal stem cells. B&auml et al. Cells, tissues, organs 2009 JAN

Abstract

Mesenchymal stem cells (MSC) can differentiate into osteoblasts, adipocytes, chondrocytes and myoblasts. It has been suggested that a reciprocal relationship exists between the differentiation of MSC into osteoblasts and adipocytes. Peroxisome proliferator-activated receptor gamma2 (PPARgamma2) is a key element for the differentiation into adipocytes. Activation of the nuclear protein deacetylase Sirt1 has recently been shown to decrease adipocyte development from preadipocytes via inhibition of PPARgamma2. In vitro, MSC differentiate to osteoblasts when exposed to bone-inducing medium. However, adipocytes are also developed. In the present study we have targeted Sirt1 to control adipocyte development during differentiation of MSC into osteoblasts. The finding that resveratrol and isonicotinamide markedly inhibited adipocyte and promoted osteoblast differentiation demonstrates an interesting alternative to PPARgamma antagonists. These results are important for the evolving field of cell-based tissue engineering, but may also be relevant in the search for new treatments of osteoporosis.
Modulation of Sirt1 by resveratrol and nicotinamide alters proliferation and differentiation of pig preadipocytes. Bai L et al. Molecular and cellular biochemistry 2008 JAN

Abstract

Sirt1, a NAD(+)-dependent histone deacetylase, may regulate senescence, metabolism, and apoptosis. In this study, primary pig preadipocytes were cultured in DMEM/F12 medium containing 10% fetal bovine serum (FBS) with or without reagents affecting Sirt1 activity. The adipocyte differentiation process was visualized by light microscopy after Oil red O staining. Proliferation and differentiation of preadipocytes was measured using methylthiazolyldiphenyl-tetrazolium bromide (MTT) and Oil red O extraction. Expression of Sirt1, FoxO1, and adipocyte specific genes was detected with semi-quantitive RT-PCR. The results showed that Sirt1 mRNA was widely expressed in various pig tissues from different developmental stages. Sirt1 mRNA was expressed throughout the entire differentiation process of pig preadipocytes. Resveratrol significantly increased Sirt1 mRNA expression, but decreased the expression of FoxO1 and adipocyte marker gene PPARgamma2. Resveratrol significantly inhibited pig preadipocyte proliferation and differentiation. Nicotinamide decreased the expression of Sirt1 mRNA, but increased the expression of FoxO1 and adipocyte specific genes. Nicotinamide greatly stimulated the proliferation and differentiation of pig preadipocytes. In conclusion, these results indicate that Sirt1 may modulate the proliferation and differentiation of pig preadipocytes. Sirt1 may down-regulate pig preadipocytes proliferation and differentiation through repression of adipocyte genes or FoxO1.

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