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STEMdiff™ Cardiomyocyte Plating Kit

A specialized media kit for improving plating efficiency post-thawing and post-harvesting of human pluripotent stem cell-derived cardiomyocytes

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STEMdiff™ Cardiomyocyte Plating Kit

A specialized media kit for improving plating efficiency post-thawing and post-harvesting of human pluripotent stem cell-derived cardiomyocytes

From: 375 USD
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A specialized media kit for improving plating efficiency post-thawing and post-harvesting of human pluripotent stem cell-derived cardiomyocytes
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Product Advantages


  • Improve plating efficiency and double the number of hPSC-derived cardiomyocytes available for your experiments

  • Retain the stable electrical profile of hPSC-derived cardiomyocytes following replating

  • Save time and money with more efficient cardiomyocyte replating after thawing and dissociation

What's Included

  • STEMdiff™ Cardiomyocyte Plating Kit (Catalog #1001121)
    • STEMdiff™ Cardiomyocyte Support Medium
    • STEMdiff™ Cardiomyocyte Plating Supplement 100X
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. STEMdiff™ Cardiomyocyte Maintenance Kit
    STEMdiff™ Cardiomyocyte Maintenance Kit

    Medium for long-term maintenance of human PSC-derived cardiomyocytes

  2. STEMdiff™ Cardiomyocyte Dissociation Kit
    STEMdiff™ Cardiomyocyte Dissociation Kit

    For dissociation of hPSC-derived cardiomyocytes

  3. D-PBS (Without Ca++ and Mg++)
    D-PBS (Without Ca++ and Mg++)

    Dulbecco’s phosphate-buffered saline without calcium and magnesium

  4. Falcon® Serological Pipettes, 10 mL
    Falcon® Serological Pipettes, 10 mL

    Sterile polystyrene pipettes for cell culture

Overview

Double the yield of human pluripotent stem cell (hPSC)-derived cardiomyocytes following dissociation, thawing, and replating by using the STEMdiff™ Cardiomyocyte Plating Kit. Consisting of the STEMdiff™ Cardiomyocyte Plating Supplement and STEMdiff™ Cardiomyocyte Support Medium, this kit improves plating efficiency and minimizes the loss of hPSC-derived cardiomyocytes.

This kit is designed for hPSC-derived cardiomyocytes generated using STEMdiff™ Ventricular Cardiomyocyte Differentiation Kit or STEMdiff™ Atrial Cardiomyocyte Differentiation Kit. By doubling your cardiomyocyte plating efficiency, the STEMdiff™ Cardiomyocyte Plating Kit can help increase your experimental throughput.

Use this kit as part of a complete, optimized workflow including these compatible STEMdiff™ cardiomyocyte products:

STEMdiff™ Ventricular Cardiomyocyte Differentiation Kit
STEMdiff™ Atrial Cardiomyocyte Differentiation Kit
STEMdiff™ Cardiomyocyte Dissociation Kit
STEMdiff™ Cardiomyocyte Support Medium
STEMdiff™ Cardiomyocyte Freezing Medium
STEMdiff™ Cardiomyocyte Maintenance Kit
Contains
serum
Cell Type
Cardiomyocytes, PSC-Derived
Species
Human
Application
Cryopreservation, Differentiation, Maintenance
Brand
STEMdiff
Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Stem Cell Biology, Cell Therapy Development

Data Figures

Graphs demonstrating iImproved plating: STEMdiff™ Cardiomyocyte Plating Medium (51%) vs. Support Medium (16%).

Figure 1. Increased Plating Efficiency Observed When Using hPSC-Derived Cardiomyocytes Using STEMdiff™ Cardiomyocyte Plating Kit

Plating efficiency of hPSC-derived cardiomyocytes was assessed 1 day after replating at low density in either STEMdiff™ Cardiomyocyte Support Medium or STEMdiff™ Cardiomyocyte Plating Medium. Across 4 hPSC lines, plating efficiency was improved when cardiomyocytes were replated in STEMdiff™ Cardiomyocyte Plating Medium (51% ± 3%, n=15) compared to STEMdiff™ Cardiomyocyte Support Medium (16% ± 1%, n=15).

Microscope images showing enhanced attachment of replated hPSC-derived cardiomyocytes using plating medium.

Figure 2. Morphology of Replated hPSC-Derived Cardiomyocytes Using STEMdiff™ Cardiomyocyte Plating Kit

Representative images of replated hPSC-derived cardiomyocytes. hPSC-derived cardiomyocytes were replated at low density in either STEMdiff™ Cardiomyocyte Support Medium or STEMdiff™ Cardiomyocyte Plating Medium. 1 day after replating, hPSC-derived cardiomyocytes exhibit improved attachment when replated with STEMdiff™ Cardiomyocyte Plating Medium.

Microelectrode array shows stable beat rate of cardiomyocytes replated with this kit.

Figure 3. hPSC-Derived Cardiomyocytes Replated Using STEMdiff™ Cardiomyocyte Plating Kit Exhibit a Stable Beat Profile

Microelectrode array recordings of hPSC-derived cardiomyocytes replated using either STEMdiff™ Cardiomyocyte Support Medium or STEMdiff™ Cardiomyocyte Plating Medium. Cardiomyocytes were maintained for 1 week with STEMdiff™ Cardiomyocyte Maintenance Medium. Across 4 hPSC lines, cardiomyocytes retain a stable beat rate when replated using STEMdiff™ Cardiomyocyte Plating Medium.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
STEMdiff™ Cardiomyocyte Plating Kit
Catalog #
100-1121
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
STEMdiff™ Cardiomyocyte Plating Kit
Catalog #
100-1121
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
STEMdiff™ Cardiomyocyte Plating Kit
Catalog #
100-1121
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (6)

Highly Efficient Single-Cell Human Pluripotent Stem Cell Cloning and Robust Cardiomyocyte Differentiation
48:54
Webinar
Highly Efficient Single-Cell Human Pluripotent Stem Cell Cloning and Robust Cardiomyocyte Differenti...
Modeling Arrhythmias Using hPSC-Derived Cardiomyocytes and Tracking Their Excitability
43:45
Webinar
Modeling Arrhythmias Using hPSC-Derived Cardiomyocytes and Tracking Their Excitability
Heterozygous Deletion of KCNH2 Models Long QT Type 2 in Human Pluripotent Stem Cell-Derived Cardiomyocytes
Scientific Poster
Heterozygous Deletion of KCNH2 Models Long QT Type 2 in Human Pluripotent Stem Cell-Derived Cardiomy...
Efficient Production and Processing of Cardiomyocytes from Human Pluripotent Stem Cells Using STEMdiff™ Cardiomyocyte Products
Scientific Poster
Efficient Production and Processing of Cardiomyocytes from Human Pluripotent Stem Cells Using STEMdi...
Rescue of Drug-Induced Long QT Syndrome Type 2 Using a hERG Channel Activator in Human Pluripotent Stem Cell-Derived Cardiomyocytes
Scientific Poster
Rescue of Drug-Induced Long QT Syndrome Type 2 Using a hERG Channel Activator in Human Pluripotent S...
Simple Label-free Monitoring of Robust Human Pluripotent Stem Cell Differentiation to Cardiomyocytes with the Maestro MEA System and STEMdiff™ Cardiomyocyte Products
Scientific Poster
Simple Label-free Monitoring of Robust Human Pluripotent Stem Cell Differentiation to Cardiomyocytes...

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Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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