R-phycoerythrin (R-PE) can be coupled to antibodies, peptides, or ligands to allow their detection by fluorescent microscopy or flow cytometry. The ID3 antibody reacts with R-PE alone or with PE-tandem dyes, such as PE-Cyanine 7, without quenching the fluorescent signal. When combined into tetrameric antibody complexes (TACs), the ID3 antibody can be used to isolate highly pure populations of PE-labeled cells.
This antibody clone is used in EasySep™ PE Positive Selection Kits (Catalog #18551 [Human], #18554 [Mouse], and #18557) and in EasySep™ Release PE Positive Selection Kits (Catalog #17654 [Human] and #17656 [Mouse]) to isolate cells of interest at high purity from single-cell suspensions derived from human, mouse, or other species. Additionally, it can be used with EasySep™ Human “Do-It-Yourself” Selection Kit (Catalog #18099) to build custom TACs for positive selection from human single-cell suspensions.