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Anti-Human CD40 Antibody, Clone 5C3

Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD40

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Anti-Human CD40 Antibody, Clone 5C3

Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD40

From: 353 USD
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Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD40
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Overview

The 5C3 antibody reacts with CD40, a 45 - 50 kDa type I transmembrane glycoprotein that is a member of the tumor necrosis factor receptor (TNFR) superfamily. CD40 plays an important role in B cell development and is also involved in the process of B cell selection in germinal centers. It facilitates T cell-B cell interactions for co-stimulation and immune regulation through engagement with CD154 (gp39), the CD40 ligand expressed on T cells. In the presence of IL-4, or other stimuli, CD40 promotes B cell proliferation, induces immunoglobulin- (Ig) isotype class switching and rescues B cells from surface Ig-induced apoptosis. CD40 is expressed on the surface of B cells, follicular dendritic cells, macrophages, fibroblasts, keratinocytes, endothelial cells, basal epithelial cells, CD34+ hematopoietic progenitor cells, and primary carcinomas. It is expressed at low levels, or not at all, on plasma cells. The 5C3 antibody has been used for the activation of B cells in in vitro functional assays or for the induction of B cell proliferation.
Subtype
Primary Antibodies
Target Antigen
CD40
Alternative Names
B cell surface antigen CD40, Bp50, CD40L receptor, CDw40, HIGM1, IGM, IMD3, Nerve growth factor receptor-related B lymphocyte activation molecule, p50, TBAM, TNFRSF5, TRAP, Tumor necrosis factor receptor superfamily member 5
Reactive Species
Baboon, Chimpanzee, Cynomolgus, Human, Rhesus, Squirrel Monkey
Conjugation
Alexa Fluor 488, APC, FITC, PE, Unconjugated
Host Species
Mouse
Cell Type
B Cells
Species
Human, Non-Human Primate
Application
Flow Cytometry, Functional Assay, Immunohistochemistry
Area of Interest
Immunology
Clone
5C3
Gene ID
958
Isotype
IgG1, kappa

Data Figures

Data for unconjugated

Figure 1. Data for Unconjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs, gated on lymphocytes) labeled with Anti-Human CD40 Antibody, Clone 5C3, followed by a rat anti-mouse IgG1 antibody, PE (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21 (Catalog #60070), followed by a rat anti-mouse IgG1 antibody, PE (solid line histogram).

Data for Alexa Fluor® 488-Conjugated

Figure 2. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs, gated on lymphocytes) labeled with Anti-Human CD40 Antibody, Clone 5C3, Alexa Fluor® 488 (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, Alexa Fluor® 488 (Catalog #60070AD, solid line histogram).

Data for APC-Conjugated

Figure 3. Data for APC-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs, gated on lymphocytes) labeled with Anti-Human CD40 Antibody, Clone 5C3, APC (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, APC (Catalog #60070AZ, solid line histogram).

Data for FITC-Conjugated

Figure 4. Data for FITC-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs, gated on lymphocytes) labeled with Anti-Human CD40 Antibody, Clone 5C3, FITC (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, FITC (Catalog #60070FI, solid line histogram).

Data for PE-Conjugated

Figure 5. Data for PE-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs, gated on lymphocytes) labeled with Anti-Human CD40 Antibody, Clone 5C3, PE (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PE (Catalog #60070PE, solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Human CD40 Antibody, Clone 5C3, APC
Catalog #
60113AZ
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD40 Antibody, Clone 5C3, PE
Catalog #
60113PE
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD40 Antibody, Clone 5C3, APC
Catalog #
60113AZ
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD40 Antibody, Clone 5C3, PE
Catalog #
60113PE
Lot #
All
Language
English

Resources and Publications

Educational Materials (2)

Reliable Antibodies for Your Research
Brochure
Reliable Antibodies for Your Research
Human Immune Cytokines
Wallchart
Human Immune Cytokines

Related Products

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Legal Statement:

Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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