Human Airway Epithelial Cell Training Course
Two-day hands-on training to support the culture of human airway epithelial cells at the air-liquid interface
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Human Airway Epithelial Cell Training Course
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Course Overview
Gain hands-on experience with the culture of human airway epithelial cells using the air-liquid interface (ALI) culture system. This system promotes extensive mucociliary differentiation of human airway epithelial cells to form a pseudostratified epithelium with morphological characteristics similar to those observed in the human airway in vivo. Participants will learn to expand primary human airway epithelial cells in PneumaCult™-Ex and PneumaCult™-Ex Plus Media, prepare cultureware for ALI culture, establish ALI cultures of human airway epithelial cells with PneumaCult™-ALI Medium, and perform downstream assays for airway epithelial cells at the ALI.
Participants Will Receive
- Course booklet and electronic versions of instructional material
- Tote bag with sample-size products (where applicable)
- Certificate following successful completion of the course
- Lunch and refreshments on each day and dinner hosted on one of the evenings
Training and Education
Training Programs
Disease Modeling; Drug Discovery and Toxicity Testing; Epithelial Cell Biology
Airway Cells
Human
Canada:
STEMCELL Technologies
1618 Station Street
Vancouver, CA V6A 1B6
STEMCELL Technologies
1618 Station Street
Vancouver, CA V6A 1B6
Speakers
Juan Hou, PhD
Senior Scientist, Research & Development
STEMCELL Technologies
STEMCELL Technologies
Guest Speaker: Details Coming Soon
Course Agenda (Tentative)
The course will end at approximately 5:00 pm on the first day and at 12:30 pm on the second day.
Day One
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Welcome Presentation and Introductions
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Lecture: Basic Biology and In Vitro Culture of Human Airway Epithelial Cells
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Practical Session: Human Airway Epithelial Cell Morphology and Maintenance
- Observe airway epithelial cells expanded in PneumaCult™ -Ex and PneumaCult™ -Ex Plus
- Observe airway epithelial cells differentiated at the air-liquid interface (ALI)
- Discuss routine maintenance of cultures
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Practical Session: Demonstration of Preparing Airway Epithelial Cells for ALI
- Demonstration of preparing cultureware
- Demonstration of harvesting and seeding of airway epithelial cells onto transwell insert
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Practical Session: Hands-On Practice with Preparing Airway Epithelial Cells for ALI
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Lecture: Assays for Human Airway Epithelial Cells
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Wrap Up and Q&A
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Course Dinner
Day Two
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Lecture: Research Applications for Human Airway Epithelial Cells
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Practical Session: Assays for Airway Epithelial Cells at the ALI (Part I)
- Demonstration & hands-on practice with transepithelial electrical resistance (TEER) readings
- Demonstration of Ussing chamber to measure epithelial membrane properties
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Practical Session: Assays for Airway Epithelial Cells at the ALI (Part II)
- Demonstration and hands-on practice with OCT and agarose embedding for histological analysis
- Demonstration of cell staining and fluorescence imaging
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Wrap up Session:
- Q&A
- Quiz
- Course Evaluation
- Certificate Presentation
Accommodations
Vancouver, CA
Pan Pacific Vancouver
999 Canada Pl #300, Vancouver, BC, Canada, V6C 3B5
Tel 1-800-663-1515 or 1-604-662-3223
Contact education@stemcell.com for information on special rates (when applicable).
Policy
Travel Visas
Citizens may require a visa in order to enter the country as visitors. Please contact us at education@stemcell.com if you require a letter of invitation for your application. Note that travel visas may take up to 30 days to process.
Travel Visa Requirements
CanadaCancellation
A 90% refund will be issued if notification is received in writing more than 30 days in advance of the course start date. No refund will be given with less than 30 days’ notice unless we are able to find another participant to fill the spot.
STEMCELL Technologies reserves the right to cancel the course if we do not receive a minimum number of registrations 30 days in advance of the start date. Should the course be cancelled, your payment will be refunded in full. We recommend that you purchase travel insurance with your flights to cover the possibility of the course being cancelled.
Scientific Resources
Educational Materials
(10)
Related Products
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Serum- and BPE-free medium for human airway epithelial cells cultured at the air-liquid interface or as airway organoids -
Serum- and BPE-free medium for expansion of primary human airway epithelial cells -
Serum- and BPE-free medium for expansion of primary human airway epithelial cells -
Cell culture supplement