Human Airway Epithelial Cell Training Course
Two-day hands-on training to support the culture of human airway epithelial cells at the air-liquid interface
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In-lab demonstration
Overview
Gain hands-on experience with the culture of human airway epithelial cells using the air-liquid interface (ALI) culture system. This system promotes extensive mucociliary differentiation of human airway epithelial cells to form a pseudostratified epithelium with morphological characteristics similar to those observed in the human airway in vivo. Participants will learn to expand primary human airway epithelial cells in PneumaCult™-Ex and PneumaCult™-Ex Plus Media, prepare cultureware for ALI culture, establish ALI cultures of human airway epithelial cells with PneumaCult™-ALI Medium, and perform downstream assays for airway epithelial cells at the ALI.
Participants Will Receive
- Course booklet and electronic versions of instructional material
- Tote bag with sample-size products (where applicable)
- Certificate following successful completion of the course
- Lunch and refreshments on each day and dinner hosted on one of the evenings
STEMCELL Technologies
1618 Station Street
Vancouver, CA V6A 1B6
Speakers
STEMCELL Technologies
Agenda
The course will end at approximately 5:00 pm on the first day and at 12:30 pm on the second day.
Day One
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Welcome Presentation and Introductions
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Lecture: Basic Biology and In Vitro Culture of Human Airway Epithelial Cells
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Practical Session: Human Airway Epithelial Cell Morphology and Maintenance
- Observe airway epithelial cells expanded in PneumaCult™ -Ex and PneumaCult™ -Ex Plus
- Observe airway epithelial cells differentiated at the air-liquid interface (ALI)
- Discuss routine maintenance of cultures
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Practical Session: Demonstration of Preparing Airway Epithelial Cells for ALI
- Demonstration of preparing cultureware
- Demonstration of harvesting and seeding of airway epithelial cells onto transwell insert
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Practical Session: Hands-On Practice with Preparing Airway Epithelial Cells for ALI
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Lecture: Assays for Human Airway Epithelial Cells
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Wrap Up and Q&A
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Course Dinner
Day Two
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Lecture: Research Applications for Human Airway Epithelial Cells
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Practical Session: Assays for Airway Epithelial Cells at the ALI (Part I)
- Demonstration & hands-on practice with transepithelial electrical resistance (TEER) readings
- Demonstration of Ussing chamber to measure epithelial membrane properties
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Practical Session: Assays for Airway Epithelial Cells at the ALI (Part II)
- Demonstration and hands-on practice with OCT and agarose embedding for histological analysis
- Demonstration of cell staining and fluorescence imaging
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Wrap up Session:
- Q&A
- Quiz
- Course Evaluation
- Certificate Presentation
Accommodation
Vancouver, CA
Contact education@stemcell.com for information on special rates (when applicable).
Policy
Travel Visas
Travel Visa Requirements
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Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (7)
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