Gentle Collagenase/Hyaluronidase

10X Gentle collagenase/hyaluronidase in DMEM

Gentle Collagenase/Hyaluronidase

10X Gentle collagenase/hyaluronidase in DMEM

From: 293 USD
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10X Gentle collagenase/hyaluronidase in DMEM
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Product Advantages


  • Gently dissociate mouse mammary tissue

  • Choose from 2-hour and overnight dissociation protocols

Overview

Perform gentle enzymatic dissociation of mouse mammary tissue with 10X Collagenase/Hyaluronidase in Dulbecco’s Modified Eagle’s Medium (DMEM). This gentle formulation enables overnight dissociation of mouse mammary gland tissue so experiments can be completed the next day rather than late at night. Gentle Collagenase/Hyluronidase has also been used for enzymatic dissociation of mouse prostate epithelial cells (Frégeau-Proulx et al. MethodsX, 2022). This protease/polysaccharidase combination digests native collagen fibrils and hydrolizes hyaluronic acids in connective tissues for effective tissue dissociation.
Contains
• 10 mg/mL Collagenase (> 1.5 U/mL)
• 1000 U/mL Hyaluronidase
• DMEM (1000 mg D-glucose/L)
Subtype
Enzymatic
Cell Type
Mammary Cells
Species
Mouse
Application
Cell Culture
Area of Interest
Epithelial Cell Biology, Stem Cell Biology

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
07919
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
07919
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications