Mouse IgM ELISA Antibody Pair Kit

For detection and measurement of mouse immunoglobulin M
Mouse IgM ELISA Antibody Pair Kit

For detection and measurement of mouse immunoglobulin M

1 Kit for 6 Plates
Catalog # 01999
443 EUR
New! For your convenience, all of our antibody pair kits now include a vial of Standard Reconstitution Buffer B. This change does not affect product performance or quality.
Required Products
  1. D-PBS Without Ca++ and Mg++
    D-PBS (Without Ca++ and Mg++)

    Dulbecco’s phosphate-buffered saline without calcium and magnesium

  2. Corning® 96-Well High-Binding Flat-Bottom Microplates
    Corning® 96-Well High-Binding Flat-Bottom Microplate

    Clear polystyrene flat-bottom, high-binding microplate without lid

  3. pNPP ELISA Substrate
    pNPP ELISA Substrate

    Substrate for ELISA antibody pair kits

Overview

The Mouse Immunoglobulin M (IgM) ELISA Antibody Pair Kit is intended for those who want the flexibility of setting up their own ELISA assay. This kit includes capture and detection antibodies and an IgM standard. It is designed for the quantitative detection and measurement of mouse IgM in biological fluids such as serum, plasma, and cell culture supernatants. IgM is predominantly found in lymph fluid and in blood where it acts as a neutralizing agent in the early stages of infection. Secreted IgM exists in a pentameric form whereas monomeric IgM is found on the surface of B cells. IgM constitutes approximately 10% of all serum immunoglobulins.
The assay is based on the sandwich ELISA method, in which samples are added to ELISA plates coated with capture antibodies specific for the immunoglobulin. The captured immunoglobulin is detected by addition of a biotinylated detection antibody, followed by streptavidin-alkaline phosphatase (SA-ALP), which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate p-nitrophenyl phosphate (pNPP) results in a colored product with an intensity directly proportional to the concentration of immunoglobulin in the sample. The concentration of the immunoglobulin is determined by comparison to a serial dilution of the immunoglobulin standard analyzed in parallel.
NOTE: This kit includes sufficient reagents for 6 x 96-well ELISA plates (Catalog #38019). ELISA plates and pNPP Substrate (Catalog #01917) are required for use with the Mouse IgM ELISA Antibody Pair Kit and are available for purchase separately.
Components
  • Mouse IgM Capture Antibody, 300 µL
  • Mouse IgM Biotinylated Detection Antibody, 80 µL
  • SA-ALP, 80 µL
  • Mouse IgM Standard, 1 vial
  • Standard Reconstitution Buffer B, 1 mL
Subtype
Antibody Pair Kits
Cell Type
B Cells, Hybridomas
Species
Mouse
Area of Interest
Hybridoma Generation, Immunology

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Mouse IgM ELISA Antibody Pair Kit
Catalog #
01999
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
Mouse IgM ELISA Antibody Pair Kit
Catalog #
01999
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
Mouse IgM ELISA Antibody Pair Kit
Catalog #
01999
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
Mouse IgM ELISA Antibody Pair Kit
Catalog #
01999
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
Mouse IgM ELISA Antibody Pair Kit
Catalog #
01999
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
Mouse IgM ELISA Antibody Pair Kit
Catalog #
01999
Lot #
All
Language
English

Educational Materials (2)

Brochure
ELISA Kits for Immunology Research
Wallchart
Human Immune Cytokines

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Representative Standard Curve

• Reportable Range: 0.1 - 25 ng/mL. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity.
• Accuracy: No international standard exists for calibration.

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