Human Recombinant NT-3

Neurotrophin-3

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Neurotrophin-3
From: 202 USD

Overview

Neurotrophin-3 (NT-3) is a neurotrophic factor and a member of the nerve growth factor (NGF) family of proteins that includes neuron growth factor (NGF), brain-derived neurotrophic factor (BDNF), and neurotrophin-4/5. NT-3 signals a number of trophic effects through its transducing receptor tyrosine kinase TrkC. NT-3 is known to promote survival, development, and differentiation of neurons, and modulates transmitter release at several types of synapses in the peripheral and central nervous systems (Chalazonitis 1996). NT-3 has been shown to have an important role in the overall development of enteric neurons, which are crucial for gut peristalsis (Chalazonitis 2004). Studies in rats have shown the potential of NT-3 in dorsal column axonal regeneration (Bradbury et al.). NT-3 was shown to protect neurons against amyloid-β toxicity (Lesne et al.). NT-3 has applications in neuronal differentiation protocols to generate β-tubulin III+ peripheral neurons from neural crest stem cells (Menendez et al.) and oligodendrocyte precursor cells from human embryonic stem (ES) and induced pluripotent stem (iPS) cells (Douvaras et al.).
Alternative Names:
HGNF, NGF-2, Nerve growth factor 2, Neurotrophin 3, NT3
Cell Type:
Neurons; Neural Stem and Progenitor Cells; Neural Cells, PSC-Derived
Species:
Human
Area of Interest:
Neuroscience
Molecular Weight:
27.5 kDa
Purity:
≥ 95%

Scientific Resources

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Educational Materials

(2)

Data and Publications

Data

(A) The biological activity of Human Recombinant NT-3 was tested by its ability to promote the proliferation of C6 cells. Cell proliferation was measured after 7 days of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 3.6 - 5.4 μg/mL. (B) 1 μg of Human Recombinant NT-3 was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant NT-3 is predicted to be a non-covalently linked homodimer, with each dimer having a molecular mass of 13.8 kDa for a predicted total molecular weight of 27.5 kDa.

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