Data and Publications
(A) The biological activity of Human Recombinant NT-3 was tested by its ability to promote the proliferation of C6 cells. Cell proliferation was measured after 7 days of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 3.6 - 5.4 μg/mL. (B) 1 μg of Human Recombinant NT-3 was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant NT-3 is predicted to be a non-covalently linked homodimer, with each dimer having a molecular mass of 13.8 kDa for a predicted total molecular weight of 27.5 kDa.