EasySep™ Mouse F4/80 Positive Selection Kit
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EasySep™ MagnetMagnet for column-free immunomagnetic separation
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EasySep™ BufferCell separation buffer
Overview
Using immunomagnetic positive selection, F4/80+ cells are labeled with antibodies and dextran-coated magnetic particles (RapidSpheres™), for column-free cell separation using an EasySep™ magnet. Isolated cells are highly purified and ready for your downstream applications.
Visit www.EasySep.com to learn more about column-free EasySep™ cell separation technology.
⦁ Up to 90% purity
⦁ No columns required
⦁ Isolated cells are not fluorochrome-labeled
- EasySep™ Mouse F4/80 Positive Selection Component A, 0.3 mL
- EasySep™ Mouse F4/80 Positive Selection Component B, 0.6 mL
- EasySep™ Dextran RapidSpheres™ 50100, 2 x 1 mL
- Normal Rat Serum, 2 mL
Scientific Resources
Product Documentation
| Document Type | Product Name | Catalog # | Lot # | Language |
|---|---|---|---|---|
|
Document Type
Product Information Sheet - Lung Tissue
|
Product Name
EasySep™ Mouse F4/80 Positive Selection Kit
|
Catalog #
100-0659 |
Lot #
All |
Language
English |
|
Document Type
Product Information Sheet - Peritoneal Lavage
|
Product Name
EasySep™ Mouse F4/80 Positive Selection Kit
|
Catalog #
100-0659 |
Lot #
All |
Language
English |
|
Document Type
Product Information Sheet - Spleen
|
Product Name
EasySep™ Mouse F4/80 Positive Selection Kit
|
Catalog #
100-0659 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 1
|
Product Name
EasySep™ Mouse F4/80 Positive Selection Kit
|
Catalog #
100-0659 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 2
|
Product Name
EasySep™ Mouse F4/80 Positive Selection Kit
|
Catalog #
100-0659 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 3
|
Product Name
EasySep™ Mouse F4/80 Positive Selection Kit
|
Catalog #
100-0659 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 4
|
Product Name
EasySep™ Mouse F4/80 Positive Selection Kit
|
Catalog #
100-0659 |
Lot #
All |
Language
English |
Educational Materials (7)
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
Data
Figure 1. Typical Profile for F4/80+ Cells Isolated from Lung Tissue Using the EasySep™ Mouse F4/80 Positive Selection Kit
Starting with a naïve mouse lung single-cell suspension, the F4/80+ cell content of the isolated fraction is typically 94.3 ± 2.8% (mean ± SD using the purple EasySep™ magnet). In the above example, the purities of the start and final isolated fractions are 26.3% and 95.3%, respectively.
Figure 2. Typical Profile for F4/80+ Cells Isolated from Peritoneal Lavage Using the EasySep™ Mouse F4/80 Positive Selection Kit
Starting with lavage cells from the peritoneal cavity of a naïve mouse, the F4/80+ cell content of the isolated fraction is typically 94.4 ± 2.9% (mean ± SD using the purple EasySep™ magnet). In the above example, the purities of the start and final isolated fractions are 40.9% and 94.1%, respectively.
Figure 3. Typical Profile for F4/80+ Cells Isolated from Spleen Using the EasySep™ Mouse F4/80 Positive Selection Kit
Starting with naïve mouse splenocytes, the F4/80+ cell content of the isolated fraction is typically 88.8 ± 3.4% (mean ± SD using the purple EasySep™ magnet). In the above example, the purities of the start and final isolated fractions are 5.4% and 90.6%, respectively.
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
