EasySep™ Mouse F4/80 Positive Selection Kit

Immunomagnetic positive selection of F4/80+ cells from mouse splenocytes, lung tissue, and peritoneal lavage
EasySep™ Mouse F4/80 Positive Selection Kit

Immunomagnetic positive selection of F4/80+ cells from mouse splenocytes, lung tissue, and peritoneal lavage

2 x 10^9 cells
Catalog # 100-0659
590 USD
Required Products
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

Overview

Easily isolate F4/80+ macrophages in as little as 24 minutes—without the use of columns—from mouse splenocytes, lung tissue, and peritoneal lavage.

Using immunomagnetic positive selection, F4/80+ cells are labeled with antibodies and dextran-coated magnetic particles (RapidSpheres™), for column-free cell separation using an EasySep™ magnet. Isolated cells are highly purified and ready for your downstream applications.

Visit www.EasySep.com to learn more about column-free EasySep™ cell separation technology.
Advantages
⦁ Fast and easy-to-use
⦁ Up to 90% purity
⦁ No columns required
⦁ Isolated cells are not fluorochrome-labeled
Components
  • EasySep™ Mouse F4/80 Positive Selection Component A, 0.3 mL
  • EasySep™ Mouse F4/80 Positive Selection Component B, 0.6 mL
  • EasySep™ Dextran RapidSpheres™ 50100, 2 x 1 mL
  • Normal Rat Serum, 2 mL
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyPlate™ EasySep™ Magnet (Catalog #18102)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
Subtype
Cell Isolation Kits
Cell Type
Macrophages
Species
Mouse
Sample Source
Lung, Other, Spleen
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep
Area of Interest
Immunology, Infectious Diseases

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet - Lung Tissue
Product Name
EasySep™ Mouse F4/80 Positive Selection Kit
Catalog #
100-0659
Lot #
All
Language
English
Document Type
Product Information Sheet - Peritoneal Lavage
Product Name
EasySep™ Mouse F4/80 Positive Selection Kit
Catalog #
100-0659
Lot #
All
Language
English
Document Type
Product Information Sheet - Spleen
Product Name
EasySep™ Mouse F4/80 Positive Selection Kit
Catalog #
100-0659
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Mouse F4/80 Positive Selection Kit
Catalog #
100-0659
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Mouse F4/80 Positive Selection Kit
Catalog #
100-0659
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Mouse F4/80 Positive Selection Kit
Catalog #
100-0659
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
EasySep™ Mouse F4/80 Positive Selection Kit
Catalog #
100-0659
Lot #
All
Language
English

Educational Materials (7)

Protocol
How to Isolate Mouse Peritoneal Cells
Wallchart
Frequencies and Percentages of Mouse Immune Cell Types
Video
How to Isolate Cells with EasySep™ Column-Free Cell Separation Technology
1:23
How to Isolate Cells with EasySep™ Column-Free Cell Separation Technology
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Webinar
Online Immunology Journal Club: New Strategies to Target Macrophages in Cancer
30:07
Online Immunology Journal Club: New Strategies to Target Macrophages in Cancer
Scientific Poster
A Serum-Free Medium for Differentiation of Monocytes to Macrophages
Scientific Poster
Easy Isolation of F4/80-Positive Macrophages from Mouse Tissues

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

FACS plot showing cell populations from lung tissue pre- and post-cell isolation using the EasySep™ Mouse F4/80 Positive Selection Kit

Figure 1. Typical Profile for F4/80+ Cells Isolated from Lung Tissue Using the EasySep™ Mouse F4/80 Positive Selection Kit

Starting with a naïve mouse lung single-cell suspension, the F4/80+ cell content of the isolated fraction is typically 94.3 ± 2.8% (mean ± SD using the purple EasySep™ magnet). In the above example, the purities of the start and final isolated fractions are 26.3% and 95.3%, respectively.

FACS plot showing cell populations from peritoneal lavage pre- and post-cell isolation using the EasySep™ Mouse F4/80 Positive Selection Kit

Figure 2. Typical Profile for F4/80+ Cells Isolated from Peritoneal Lavage Using the EasySep™ Mouse F4/80 Positive Selection Kit

Starting with lavage cells from the peritoneal cavity of a naïve mouse, the F4/80+ cell content of the isolated fraction is typically 94.4 ± 2.9% (mean ± SD using the purple EasySep™ magnet). In the above example, the purities of the start and final isolated fractions are 40.9% and 94.1%, respectively.

FACS plot showing cell populations from spleen pre- and post-cell isolation using the EasySep™ Mouse F4/80 Positive Selection Kit

Figure 3. Typical Profile for F4/80+ Cells Isolated from Spleen Using the EasySep™ Mouse F4/80 Positive Selection Kit

Starting with naïve mouse splenocytes, the F4/80+ cell content of the isolated fraction is typically 88.8 ± 3.4% (mean ± SD using the purple EasySep™ magnet). In the above example, the purities of the start and final isolated fractions are 5.4% and 90.6%, respectively.

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