EasySep™ Mouse CD138 Positive Selection Kit
Immunomagnetic positive selection kit
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EasySep™ Mouse CD138 Positive Selection Kit -
EasySep™ Mouse CD138 Positive Selection Kit -
Label for EasySep™ Mouse CD138 Positive Selection Kit
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Required Products
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Magnet for column-free immunomagnetic separation -
Cell separation buffer -
Compatible antibodies for purity assessment of isolated cells
Overview
The EasySep™ Mouse CD138 Positive Selection Kit is designed to isolate CD138+ cells from mouse splenocytes, lymph nodes and bone marrow by immunomagnetic positive selection. Desired cells are targeted with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Isolated cells are immediately available for downstream applications such as flow cytometry, cell culture, and hybridoma generation.
Advantages:
• Fast, easy-to-use and column-free
• Up to 97% purity with immunized mice
• Useful for the enrichment of plasma cells
• Compatible with hybridoma generation protocols, including electrofusion
• Up to 97% purity with immunized mice
• Useful for the enrichment of plasma cells
• Compatible with hybridoma generation protocols, including electrofusion
Components:
- EasySep™ Mouse CD138 Positive Selection Kit (Catalog #18957)
- EasySep™ Mouse CD138 Positive Selection Cocktail, 1 mL
- EasySep™ Dextran RapidSpheres™ 50100, 1 mL
- EasySep™ Mouse FcR Blocker, 2 x 0.5 mL
- RoboSep™ Mouse CD138 Positive Selection Kit (Catalog #18957RF)
- EasySep™ Mouse CD138 Positive Selection Cocktail, 1 mL
- EasySep™ Dextran RapidSpheres™ 50100, 1 mL
- EasySep™ Mouse FcR Blocker, 2 x 0.5 mL
- RoboSep™ Buffer (Catalog #20104)
- RoboSep™ Filter Tips (Catalog #20125)
- EasySep™ EasyTube™-14 (Catalog #20128) x 2
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype:
Cell Isolation Kits
Cell Type:
B Cells; Plasma
Species:
Mouse
Sample Source:
Bone Marrow; Lymph Node; Other; Spleen
Selection Method:
Positive
Application:
Cell Isolation
Brand:
EasySep; RoboSep
Area of Interest:
Immunology
Related Products
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EasySep™ Mouse CD19 Positive Selection Kit II
Immunomagnetic positive selection kit -
ClonaCell™-HY AOF Expansion Medium
Hybridoma expansion medium with hypoxanthine and thymidine (animal origin-free) -
EasySep™ Mouse B Cell Isolation Kit
15-Minute cell isolation kit using immunomagnetic negative selection
Labeling Antibodies
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Anti-Mouse CD45R (B220) Antibody, Clone RA3-6B2
Rat monoclonal IgG2a antibody against human, mouse, cat CD45R (B220) -
Anti-Mouse CD138 (Syndecan-1) Antibody, Clone 281-2
Rat monoclonal IgG2a antibody against mouse CD138 (syndecan-1) -
Anti-Mouse CD267 (TACI) Antibody, Clone 8F10
Rat monoclonal IgG2a antibody against mouse CD267 (TACI)
Scientific Resources
Product Documentation
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Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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Data
Figure 1. Typical EasySep™ Mouse CD138 Positive Selection Kit Isolation Profile
(A) Starting with immunized BALB/c mouse splenocytes, the CD138+ cell content of the isolated fraction is typically 81.5 ± 4.9% (mean ± SD). In the above example, the purities of the start and final isolated fractions are 4.6% and 83.3%, respectively. (B) Starting with immunized BALB/c mouse splenocytes, the plasma cell (CD138+CD267 (TACI)+) content is typically 68.5 ± 11.3% (mean ± SD). In the above example, the purities of the start and final isolated fractions are 1.3% and 70.2%, respectively. (C) Starting with naïve C57BL/6 mouse splenocytes, the CD138+ cell content of the isolated fraction is typically 78.3 ± 5.7% (mean ± SD). In the above example, the purities of the start and final isolated fractions are 5.0% and 79.5%, respectively. (D) Starting with naïve C57BL/6 mouse splenocytes, the plasma cell (CD138+CD267 (TACI)+) content is typically 50.8 ± 10.0 % (mean ± SD). In the above example, the purities of the start and final isolated fractions are 0.4% and 56.3%, respectively. (E) Isolated CD138+ cells or total splenocytes from mice immunized with various antigens were fused with Sp2/0 mouse myeloma cells and plated in semi-solid medium using ClonaCell™-HY Hybridoma Kit (Catalog #03800). The % antigen-specific hit rate was determined by ELISA. The % antigen-specific hit rates for total splenocytes and CD138+ cells were 4.1 ± 1.6% and 28.8 ± 11.0% (mean ± SD), respectively. (F) Isolated CD138+ cells or total splenocytes from mice immunized with various antigens were fused with Sp2/0 mouse myeloma cells and plated in semi-solid medium using ClonaCell™-HY Hybridoma Kit (Catalog #03800). The % antibody-secreting hybridomas was determined by ELISA. The % antibody-secreting hybridomas for total splenocytes and CD138+ cells were 33.0 ± 8.7% and 99.3 ± 0.6% (mean ± SD), respectively.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.