Overview
• Up to 97% purity with immunized mice
• Useful for the enrichment of plasma cells
• Compatible with hybridoma generation protocols, including electrofusion
- EasySep™ Mouse CD138 Positive Selection Kit (Catalog #18957)
- EasySep™ Mouse CD138 Positive Selection Cocktail, 1 mL
- EasySep™ Dextran RapidSpheres™ 50100, 1 mL
- EasySep™ Mouse FcR Blocker, 2 x 0.5 mL
- RoboSep™ Mouse CD138 Positive Selection Kit (Catalog #18957RF)
- EasySep™ Mouse CD138 Positive Selection Cocktail, 1 mL
- EasySep™ Dextran RapidSpheres™ 50100, 1 mL
- EasySep™ Mouse FcR Blocker, 2 x 0.5 mL
- RoboSep™ Buffer (Catalog #20104)
- RoboSep™ Filter Tips (Catalog #20125)
- EasySep™ EasyTube™-14 (Catalog #20128) x 2
Scientific Resources
Product Documentation
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(12)Product Applications
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Data and Publications
Data

Figure 1. Typical EasySep™ Mouse CD138 Positive Selection Kit Isolation Profile
(A) Starting with immunized BALB/c mouse splenocytes, the CD138+ cell content of the isolated fraction is typically 81.5 ± 4.9% (mean ± SD). In the above example, the purities of the start and final isolated fractions are 4.6% and 83.3%, respectively. (B) Starting with immunized BALB/c mouse splenocytes, the plasma cell (CD138+CD267 (TACI)+) content is typically 68.5 ± 11.3% (mean ± SD). In the above example, the purities of the start and final isolated fractions are 1.3% and 70.2%, respectively. (C) Starting with naïve C57BL/6 mouse splenocytes, the CD138+ cell content of the isolated fraction is typically 78.3 ± 5.7% (mean ± SD). In the above example, the purities of the start and final isolated fractions are 5.0% and 79.5%, respectively. (D) Starting with naïve C57BL/6 mouse splenocytes, the plasma cell (CD138+CD267 (TACI)+) content is typically 50.8 ± 10.0 % (mean ± SD). In the above example, the purities of the start and final isolated fractions are 0.4% and 56.3%, respectively. (E) Isolated CD138+ cells or total splenocytes from mice immunized with various antigens were fused with Sp2/0 mouse myeloma cells and plated in semi-solid medium using ClonaCell™-HY Hybridoma Kit (Catalog #03800). The % antigen-specific hit rate was determined by ELISA. The % antigen-specific hit rates for total splenocytes and CD138+ cells were 4.1 ± 1.6% and 28.8 ± 11.0% (mean ± SD), respectively. (F) Isolated CD138+ cells or total splenocytes from mice immunized with various antigens were fused with Sp2/0 mouse myeloma cells and plated in semi-solid medium using ClonaCell™-HY Hybridoma Kit (Catalog #03800). The % antibody-secreting hybridomas was determined by ELISA. The % antibody-secreting hybridomas for total splenocytes and CD138+ cells were 33.0 ± 8.7% and 99.3 ± 0.6% (mean ± SD), respectively.