EasySep™ Mouse CD138 Positive Selection Kit

Immunomagnetic positive selection kit

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From: 637 USD

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Immunomagnetic positive selection kit
From: 637 USD

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Overview Related Products Scientific Resources Product Applications Data and Publications

Overview

The EasySep™ Mouse CD138 Positive Selection Kit is designed to isolate CD138+ cells from mouse splenocytes, lymph nodes and bone marrow by immunomagnetic positive selection. Desired cells are targeted with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Isolated cells are immediately available for downstream applications such as flow cytometry, cell culture, and hybridoma generation.
Advantages:
• Fast, easy-to-use and column-free
• –Up to 97% purity with immunized mice
• Useful for the enrichment of plasma cells
• Compatible with hybridoma generation protocols, including electrofusion
Components:
  • EasySep™ Mouse CD138 Positive Selection Kit (Catalog #18957)
    • EasySep™ Mouse CD138 Positive Selection Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 1 mL
    • EasySep™ Mouse FcR Blocker, 2 x 0.5 mL
  • RoboSep™ Mouse CD138 Positive Selection Kit (Catalog #18957RF)
    • EasySep™ Mouse CD138 Positive Selection Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 1 mL
    • EasySep™ Mouse FcR Blocker, 2 x 0.5 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
    • EasySep™ EasyTube™-14 (Catalog #20128) x 2
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype:
Cell Isolation Kits
Cell Type:
B Cells; Plasma Cells
Species:
Mouse
Sample Source:
Bone Marrow; Lymph Node; Other; Spleen
Selection Method:
Positive
Application:
Cell Isolation
Brand:
EasySep; RoboSep
Area of Interest:
Immunology

Scientific Resources

Product Documentation

Educational Materials

(12)
Brochure
ClonaCell™ Media for Hybridoma and Cell Line Development
Brochure
Increase the Efficiency of Your Hybridoma Workflow with EasySep™ and ClonaCell™
Brochure
EasySep™ Cell Separation Technology
Brochure
Tools For Your Immunology Research
Wallchart
Frequencies and Percentages of Mouse Immune Cell Types
Video
8:24
ClonaCell™-HY Procedure
Video
1:41
RoboSep™-S Introduction
Video
1:13
The EasySep™ Pour-off: A Simple and Effective Way to Isolate Cells
Video
0:57
EasyEights™: Faster and Easier Multiple Sample Cell Isolations
Video
1:57
Fast and Easy Cell Isolation with EasySep™
Scientific Poster
The Hybridoma Workflow is Improved by Incorporating CD138+ Cell Selection, B Cell Activation and Expansion, and Serum-Free Culture
Scientific Poster
EasySep™ Kits for Immunomagnetic Purification of CD138+ cells from Mouse and Rat Samples

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area Workflow Stages for
Workflow Stages
B Cell Research
Cell Sourcing
Cell Isolation
Cell Characterization
Hybridoma Production
Pre-Enrichment and Fusion
Selection and Cloning
Expansion and Characterization

Data and Publications

Data

Figure 1. Typical EasySep™ Mouse CD138 Positive Selection Kit Isolation Profile

(A) Starting with immunized BALB/c mouse splenocytes, the CD138+ cell content of the isolated fraction is typically 81.5 ± 4.9% (mean ± SD). In the above example, the purities of the start and final isolated fractions are 4.6% and 83.3%, respectively. (B) Starting with immunized BALB/c mouse splenocytes, the plasma cell (CD138+CD267 (TACI)+) content is typically 68.5 ± 11.3% (mean ± SD). In the above example, the purities of the start and final isolated fractions are 1.3% and 70.2%, respectively. (C) Starting with naïve C57BL/6 mouse splenocytes, the CD138+ cell content of the isolated fraction is typically 78.3 ± 5.7% (mean ± SD). In the above example, the purities of the start and final isolated fractions are 5.0% and 79.5%, respectively. (D) Starting with naïve C57BL/6 mouse splenocytes, the plasma cell (CD138+CD267 (TACI)+) content is typically 50.8 ± 10.0 % (mean ± SD). In the above example, the purities of the start and final isolated fractions are 0.4% and 56.3%, respectively. (E) Isolated CD138+ cells or total splenocytes from mice immunized with various antigens were fused with Sp2/0 mouse myeloma cells and plated in semi-solid medium using ClonaCell™-HY Hybridoma Kit (Catalog #03800). The % antigen-specific hit rate was determined by ELISA. The % antigen-specific hit rates for total splenocytes and CD138+ cells were 4.1 ± 1.6% and 28.8 ± 11.0% (mean ± SD), respectively. (F) Isolated CD138+ cells or total splenocytes from mice immunized with various antigens were fused with Sp2/0 mouse myeloma cells and plated in semi-solid medium using ClonaCell™-HY Hybridoma Kit (Catalog #03800). The % antibody-secreting hybridomas was determined by ELISA. The % antibody-secreting hybridomas for total splenocytes and CD138+ cells were 33.0 ± 8.7% and 99.3 ± 0.6% (mean ± SD), respectively.

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.