EasySep™ Human ILC2 Isolation Kit

Immunomagnetic positive selection kit using particle release technology

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EasySep™ Human ILC2 Isolation Kit

Immunomagnetic cell isolation kit using particle release technology

2 x 109 cells
Catalog #17782
994 USD

Required Products

Overview

The EasySep™ Human ILC2 Isolation Kit is designed to isolate Group 2 Innate Lymphoid Cells (ILC2s) from leukapheresis products by immunomagnetic positive selection without the use of a flow sorter.

First, CRTH2+ cells are isolated by column-free immunomagnetic positive selection using antibody complexes and EasySep™ Releasable RapidSpheres™. Then, bound magnetic particles are removed from the EasySep™-isolated CRTH2+ cells, and unwanted non-ILC2s are targeted for depletion using antibody complexes and EasySep™ Dextran RapidSpheres™. The final isolated fraction contains highly purified ILC2s that are immediately ready for downstream applications.

Following cell isolation with this EasySep™ kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands.
Advantages:
• Easy-to-use and column-free
• Up to 95% purity
• Facilitates rapid isolation of ILC2s without flow sorting
• No-wash removal of EasySep™ Releasable RapidSpheres™
Components:
  • EasySep™ Human ILC2 Isolation Kit (Catalog #17782)
    • EasySep™ Human CRTH2-PE Positive Selection Cocktail, 0.1 mL
    • EasySep™ Release PE Positive Selection Cocktail, 2 x 0.5mL
    • EasySep™ Releasable RapidSpheres™ 50201, 1 mL
    • EasySep™ Release Buffer, 1 mL
    • EasySep™ Human ILC2 Isolation Cocktail, 0.5 mL
    • EasySep™ Dextran RapidSpheres™ 50103, 1 mL
    • Anti-Human CD32 Blocker, 1 mL
 
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
 
Subtype:
Cell Isolation Kits
Cell Type:
Innate Lymphoid Cells
Species:
Human
Sample Source:
Leukapheresis; PBMC
Selection Method:
Positive
Application:
Cell Isolation
Brand:
EasySep
Area of Interest:
Immunology

Scientific Resources

Educational Materials

(9)

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data


Figure 1. Typical EasySep™ Human ILC2 Isolation Profile

Starting with fresh leukapheresis samples, the ILC2 content (CD45+Lin- CRTH2+CD161+CD127+) of the isolated fraction typically ranges from 84 - 95%. In the above example, the purities of the start and final isolated fractions are 0.03% and 91% (gated on CD45), respectively.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.