EasySep™ Human B Cell Enrichment Kit II Without CD43 Depletion

Immunomagnetic negative selection kit

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From: 730 USD

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Immunomagnetic negative selection kit
From: 730 USD

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Overview

The EasySep™ Human B Cell Enrichment Kit II without CD43 Depletion is designed to isolate B cells by negative selection from fresh or previously frozen PBMCs of individuals with B cell leukemia or lymphoma, or other disease states in which B cells may express CD43. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.

This kit replaces the EasySep™ Human B Cell Enrichment Kit without CD43 (Catalog #19154) for even faster cell isolations.
Advantages:
• Fast and easy-to-use
• Up to 99% purity
• Isolated cells are untouched
Components:
  • EasySep™ Human B Cell Enrichment Kit II without CD43 Depletion (Catalog #17963)
    • EasySep™ Human B Cell Enrichment Cocktail II without CD43 Depletion, 1 mL
    • EasySep™ Human Isolation Cocktail Enhancer, 1 mL
    • EasySep™ Dextran RapidSpheres™, 1 mL
  • RoboSep™ Human B Cell Enrichment Kit II without CD43 Depletion (Catalog #17963RF)
    • EasySep™ Human B Cell Enrichment Cocktail II without CD43 Depletion, 1 mL
    • EasySep™ Human Isolation Cocktail Enhancer, 1 mL
    • EasySep™ Dextran RapidSpheres™, 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype:
Cell Isolation Kits
Cell Type:
B Cells
Species:
Human
Sample Source:
PBMC
Selection Method:
Negative
Application:
Cell Isolation
Brand:
EasySep; RoboSep
Area of Interest:
Immunology

Scientific Resources

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Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Starting with fresh PBMCs, the B cell content (CD19+) of the enriched fraction is typically 84.9 ± 13.9% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final enriched fractions are 14.8% and 85.8%, respectively.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.