MegaCult™-C Collagen and Medium Without Cytokines
Collagen and medium without cytokines for human and mouse CFU-Mk assays
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Overview
• MegaCult™-C Medium Without Cytokines is intended for the culture of CFU-Mk in human bone marrow, mobilized peripheral blood, and cord blood samples, after addition of appropriate cytokines. It is suitable for use with CD34 + enriched cells, mononuclear cells, and cells isolated by other purification methods. It is also intended for assays of megakaryocyte progenitor cells in unseparated or purified cell suspensions of mouse bone marrow, after addition of appropriate cytokines.
• Collagen Solution for preparation of collagen gels and for coating cell culture surfaces.
Individual kit components may also be purchased separately, for your convenience.
For more information on protocols for human CFU-Mk assays using MegaCult™-C, please explore the Technical Manual.
Data Figures

Figure 1. Procedure Summary for Assays of Human Megakaryocytic Progenitors

Figure 2. Procedure Summary for Assays of Mouse Megakaryocytic Progenitors

Figure 3. Examples of Colonies Derived From Human Megakaryocyte Progenitors
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (2)
Frequently Asked Questions
Why is the MegaCult™-C formulation serum free?
Why use semi-solid media?
Publications (30)
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Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.