ClonaCell™ FLEX

Semi-solid methylcellulose-based medium for selecting and cloning cells
ClonaCell™ FLEX

Semi-solid methylcellulose-based medium for selection and cloning of cells

45 mL
Catalog # 03818
288 USD

Overview

ClonaCell™ FLEX is a base methylcellulose medium that requires the addition of a 2X concentrate of the user’s own liquid medium to create a custom semi-solid cloning medium. This allows the user to adapt an existing liquid cloning method to a customized semi-solid cloning method. ClonaCell™ FLEX is recommended for selection and cloning of suspension-adapted mammalian cells, including CHO cells and hybridomas. The medium is chemically defined, animal component-free, and protein-free. It does not contain L-glutamine, selection agents, or phenol red. ClonaCell™ FLEX is compatible with a wide range of selection systems, including dihydrofolate reductase (DHFR), glutamine synthetase (GS), and hypoxanthine, aminopterin, thymidine (HAT).

Benefits of semi-solid cloning:
• Individual clones and their progeny remain localized together in a semi-solid matrix as they grow to form distinct colonies. This prevents the loss of rare, high-producing clones by overgrowth from faster-growing cells, as can occur during selection in a liquid medium, and facilitates the isolation of a diverse set of clones with a wide range of growth rates and productivities to be obtained for downstream screening.
• Colonies obtained from semi-solid medium have a high probability of monoclonality, allowing clonal cell lines to be generated in less time and using fewer resources than with limiting dilution cloning.
• Colonies can be easily picked from the semi-solid medium by manual or robotic methods and dispersed into a liquid growth medium for screening and expansion.
Advantages
• Allows the user to convert an existing liquid cloning method into a semi-solid cloning method using a 2X concentrate of their liquid medium of choice
• Flexibility to create a custom semi-solid medium optimized for cloning a wide variety of mammalian or non-mammalian cell types
Contains
• Methylcellulose
• Water
Subtype
Semi-Solid Media, Specialized Media
Cell Type
CHO Cells, HEK-293, Hybridomas, Other
Species
Mouse, Other
Application
Cell Culture, Semi-Solid Cloning
Brand
ClonaCell
Area of Interest
Antibody Development, Cell Line Development, Drug Discovery and Toxicity Testing, Hybridoma Generation
Formulation
Animal Component-Free, Serum-Free, Chemically Defined, Protein-Free

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
ClonaCell™ FLEX
Catalog #
03818
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
ClonaCell™ FLEX
Catalog #
03818
Lot #
All
Language
English

Educational Materials (12)

Brochure
ClonaCell™ Media for Hybridoma and Cell Line Development
Brochure
ClonaCell-CHO Semi-Solid Cloning Testing Guidelines
Technical Bulletin
Protocol for Producing Monoclonal Cell Lines Using ClonaCell™ FLEX Semi-Solid Medium
Video
How to Generate Hybridomas in 96-Well Plates Using ClonaCell™-HY Semi-Solid Cloning Medium
5:56
How to Generate Hybridomas in 96-Well Plates Using ClonaCell™-HY Semi-Solid Cloning Medium
Video
Semi-Solid Cloning of CHO Cells Using ClonaCell™-CHO Medium
10:50
Semi-Solid Cloning of CHO Cells Using ClonaCell™-CHO Medium
Video
Why Use Semi-Solid Medium for Cloning Hybridomas and CHO Cells
3:39
Why Use Semi-Solid Medium for Cloning Hybridomas and CHO Cells
Video
ClonaCell™-HY Procedure
8:24
ClonaCell™-HY Procedure
Video
Video Tutorial: Probability of Monoclonality
21:01
Video Tutorial: Probability of Monoclonality
Video
Mammalian Cell Cloning Procedure: Customizable Semi-Solid Cloning with ClonaCell™ FLEX Medium
9:45
Mammalian Cell Cloning Procedure: Customizable Semi-Solid Cloning with ClonaCell™ FLEX Medium
Video
Customizable Semi-Solid Cloning of Mammalian Cells in 96-Well Plates Using ClonaCell™ FLEX Medium
12:46
Customizable Semi-Solid Cloning of Mammalian Cells in 96-Well Plates Using ClonaCell™ FLEX Medium
Video
How to Perform Semi-Solid Cloning of CHO Cell Lines in 96-Well Plates Using ClonaCell™-CHO Medium
12:47
How to Perform Semi-Solid Cloning of CHO Cell Lines in 96-Well Plates Using ClonaCell™-CHO Medium
Webinar
A Smarter Way To Clone: What You Don't Know About Limiting Dilution Can Hurt Your Probability of Monoclonality
34:50
A Smarter Way To Clone: What You Don't Know About Limiting Dilution Can Hurt Your Probability of Monoclonality

Frequently Asked Question

Is the serum in ClonaCell™-TCS medium heat inactivated?

Yes, all serum used in ClonaCell™ is heat inactivated.

Is there any IgG in ClonaCell™ TCS?

While we don't add IgG to the ClonaCell™ media, we do add serum, which contains an undefined amount of IgG. We selectively use serum lots with low IgG levels in the production of ClonaCell™ media, however, levels vary from lot to lot. IgG levels in a specific lot of ClonaCell™ TCS medium are available in the lot-specific Certificate of Analysis.

Can ClonaCell™-TCS be used with any cell line?

A list of recommended cell lines can be found in the manual. Other cell lines may be compatible with ClonaCell™-TCS. It will be necessary, however, to determine the plating cell density and growth efficiency of the desired cells in ClonaCell™-TCS.
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Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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