Anti-Mouse SSEA-1 (CD15) Antibody, Clone MC-480
Mouse monoclonal IgM antibody against human, mouse, rat SSEA-1 (CD15)
Overview
This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ HLA Whole Blood CD15 Positive Selection Kit (Catalog #18681HLA; partial blocking may be observed), and for labeling human ES and iPS cells grown in TeSR™-E8™ (Catalog #05940), mTeSR™1 (Catalog #85850) and TeSR™2 (Catalog #05860).
Data Figures
Figure 1. Data for Alexa Fluor® 488-Conjugated
(A) Flow cytometry analysis of human whole blood nucleated cells labeled with Anti-Human SSEA-1 (CD15) Antibody, Clone MC-480, Alexa Fluor® 488
(filled histogram) or Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, Alexa Fluor® 488 (Catalog #60069AD) (solid line histogram). SSEA-1 is
highly expressed on granulocytes.
(B) Flow cytometry analysis of human HT1080 fibrosarcoma cells labeled with Anti-Human SSEA-1 (CD15) Antibody, Clone MC-480, Alexa Fluor® 488
(filled histogram). Labeling of human HT1080 fibrosarcoma (solid line histogram) or ES cells (negative control; dashed line histogram) with a mouse IgM, kappa isotype control antibody (Anti-Human TRA-1-60 Antibody, Clone TRA-1-60R, Alexa Fluor® 488; Catalog #60064AD) is shown. SSEA-1 is not
expressed on undifferentiated human ES cells.
(C) Human ES cells were cultured in mTeSR™1 on Corning® Matrigel®-coated glass slides, then fixed and labeled with Anti-Human SSEA-1 (CD15)
Antibody, Clone MC-480, Alexa Fluor® 488. Inset shows cells labeled with Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, Alexa Fluor® 488.
Figure 2. Data for Biotin-Conjugated
(A) Flow cytometry analysis of human buffy coat nucleated cells labeled with Anti-Human SSEA-1 Antibody, Clone MC-480, Biotin, followed by streptavidin (SAV) APC (filled histogram), or a mouse IgM, kappa biotin isotype control antibody, followed by SAV APC (solid line histogram). (B) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Whole Blood CD15 Positive Selection Kit and labeled with Anti-Human SSEA-1 Antibody, Clone MC-480, Biotin, followed by SAV APC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling with a mouse IgM, kappa biotin isotype control antibody, followed by SAV APC is shown (solid line histogram).
Figure 3. Data for FITC-Conjugated
(A) Flow cytometry analysis of human buffy coat nucleated cells labeled with Anti-Human SSEA-1 Antibody, Clone MC-480, FITC (filled histogram) or a mouse IgM, kappa FITC Isotype control antibody (solid line histogram). SSEA-1 (CD15) is highly expressed on granulocytes. (B) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA CD15 WB Positive Selection Kit and labeled with Anti-Human SSEA-1 Antibody, Clone MC-480, FITC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgM, kappa FITC Isotype control antibody is shown (solid line histogram).
Figure 4. Data for PE-Conjugated
(A) Flow cytometry analysis of human whole blood nucleated cells labeled with Anti-Human SSEA-1 (CD15) Antibody, Clone MC-480, PE (filled histogram)
or Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, PE (Catalog #60069PE) (solid line histogram). SSEA-1 is highly expressed on granulocytes.
(B) Human ES cells were cultured in mTeSR™1 on Corning® Matrigel®-coated glass slides, then fixed and labeled with Anti-Human SSEA-1 (CD15)
Antibody, Clone MC-480, PE. Inset shows cells labeled with Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, PE. SSEA-1 is not expressed on
undifferentiated human ES cells.
(C) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA CD15 WB Positive Selection Kit and labeled with
Anti-Human SSEA-1 (CD15) Antibody, Clone MC-480, PE. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated).
Labeling with Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, PE is shown (solid line histogram).
Figure 5. Data for Unconjugated
(A) Flow cytometry analysis of human whole blood nucleated cells labeled with Anti-Human SSEA-1 (CD15) Antibody, Clone MC-480, followed by Goat
Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or Mouse IgM, kappa Isotype Control Antibody, Clone MM-30
(Catalog #60069), followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram). SSEA-1 is highly expressed on granulocytes.
(B) Flow cytometry analysis of human HT1080 fibrosarcoma cells labeled with Anti-Human SSEA-1 (CD15) Antibody, Clone MC-480, followed by goat antimouse
IgG, FITC (filled histogram). Labeling of human HT1080 fibrosarcoma cells (solid line histogram) or H1 ES cells (negative control; dashed line
histogram) with a mouse IgM, kappa isotype control antibody (Anti-Human TRA-1-60 Antibody, Clone TRA-1-60R; Catalog #60064) is shown.
(C) Human ES cells were cultured in mTeSR™1 on Corning® Matrigel®-coated glass slides, then fixed and labeled with Anti-Human SSEA-1 (CD15)
Antibody, Clone MC-480, followed by goat anti-mouse IgG, FITC. Inset shows cells labeled with Mouse IgM, kappa Isotype Control Antibody, Clone MM-
30, followed by goat anti-mouse IgG, FITC. SSEA-1 is not expressed on undifferentiated human ES cells.
(D) Western blot analysis of denatured/reduced cell lysates from human ES cells (negative control; lane 1) or HT1080 fibrosarcoma cells (lane 2) with
Anti-Human SSEA-1 (CD15) Antibody, Clone MC-480.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (3)
Publications (1)
Abstract
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Legal Statement:
Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
