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Anti-Human Epithelial Cell Antibody, Clone 5E11.3.1

Mouse monoclonal IgG1 antibody against human epithelial cells

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Anti-Human Epithelial Cell Antibody, Clone 5E11.3.1

Mouse monoclonal IgG1 antibody against human epithelial cells

From: 444 USD
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Mouse monoclonal IgG1 antibody against human epithelial cells
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Overview

The 5E11.3.1 antibody reacts with an unidentified marker expressed on the surface of most luminal cells of normal human mammary epithelium. Weak labeling (or no labeling) is observed within the basal cell compartment of the mammary epithelium. The 5E11.3.1 antibody gives variable labeling on malignant breast epithelium. Positive labeling is observed on the following cell lines: MCF7, CAMA, T47D, BT-10, BT-20, COLO 205, and WiDr. Labeling is negative on human bone marrow cells, blood cells, lymphoid and mesenchymal tissues, mammary and marrow fibroblast cultures, and human embryonic stem cell line H9.
Subtype
Primary Antibodies
Target Antigen
Human Epithelial Cell
Alternative Names
Not applicable
Reactive Species
Human
Conjugation
FITC, Unconjugated
Host Species
Mouse
Cell Type
Mammary Cells
Species
Human
Application
Cell Isolation, Flow Cytometry, Immunohistochemistry
Area of Interest
Epithelial Cell Biology, Stem Cell Biology
Clone
5E11.3.1
Gene ID
4072
Isotype
IgG1, kappa

Data Figures

Figure 1. Data for Unconjugated

Flow cytometry analysis of human MCF7 cells labeled with Anti-Human Epithelial Cell Antibody, Clone 5E11.3.1, followed by Goat Anti-Mouse IgG Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21 (Catalog #60070), followed by Goat Anti-Mouse IgG Antibody, Polyclonal, FITC (solid line histogram).

Figure 2. Data for FITC-conjugated

Flow cytometry analysis of human MCF7 cells labeled with Anti-Human Epithelial Cell Antibody, Clone 5E11.3.1, FITC (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, FITC (Catalog #60070FI) (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Human Epithelial Cell Antibody, Clone 5E11.3.1
Catalog #
60147
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human Epithelial Cell Antibody, Clone 5E11.3.1, FITC
Catalog #
60147FI
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human Epithelial Cell Antibody, Clone 5E11.3.1
Catalog #
60147
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human Epithelial Cell Antibody, Clone 5E11.3.1, FITC
Catalog #
60147FI
Lot #
All
Language
English

Resources and Publications

Educational Materials (1)

Reliable Antibodies for Your Research
Brochure
Reliable Antibodies for Your Research

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Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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