Anti-Human Epithelial Cell Antibody, Clone 5E11.3.1

Mouse monoclonal IgG1 antibody against human epithelial cells

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Mouse monoclonal IgG1 antibody against human epithelial cells
From: 318 USD

Overview

The 5E11.3.1 antibody reacts with an unidentified marker expressed on the surface of most luminal cells of normal human mammary epithelium. Weak labeling (or no labeling) is observed within the basal cell compartment of the mammary epithelium. The 5E11.3.1 antibody gives variable labeling on malignant breast epithelium. Positive labeling is observed on the following cell lines: MCF7, CAMA, T47D, BT-10, BT-20, COLO 205, and WiDr. Labeling is negative on human bone marrow cells, blood cells, lymphoid and mesenchymal tissues, mammary and marrow fibroblast cultures, and human embryonic stem cell line H9.
Subtype:
Primary Antibodies
Target Antigen:
CD326 (EpCAM)
Alternative Names:
Not applicable
Reactive Species:
Human
Conjugation:
FITC; Unconjugated
Host Species:
Mouse
Cell Type:
Mammary Cells
Application:
Cell Isolation; Flow Cytometry; Immunohistochemistry
Area of Interest:
Epithelial Cell Biology; Stem Cell Biology
Clone:
5E11.3.1
Gene ID:
4072
Isotype:
IgG1, kappa

Scientific Resources

Educational Materials

(2)

Data and Publications

Data

Figure 1. Data for Unconjugated

Flow cytometry analysis of human MCF7 cells labeled with Anti-Human Epithelial Cell Antibody, Clone 5E11.3.1, followed by Goat Anti-Mouse IgG Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21 (Catalog #60070), followed by Goat Anti-Mouse IgG Antibody, Polyclonal, FITC (solid line histogram).

Figure 2. Data for FITC-conjugated

Flow cytometry analysis of human MCF7 cells labeled with Anti-Human Epithelial Cell Antibody, Clone 5E11.3.1, FITC (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, FITC (Catalog #60070FI) (solid line histogram).

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.