Human IL-10 ELISA Kit
For detection and measurement of human interleukin 10
The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for the cytokine. The captured cytokine is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase, which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate 3,3’,5,5’ tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of cytokine in the sample. The concentration of the cytokine is determined by comparison to a serial dilution of the cytokine standard analyzed in parallel.
Representative Standard Curve
• Reportable range: 3.2 - 1000 pg/mL. This is the concentration range in which measurement of the analyte can be done with the highest
precision, accuracy, and linearity.
• Sensitivity: The limit of detection of this assay is 0.5 pg/mL. This is the analyte concentration with absorbance two standard deviations higher than the zero standard.
• Accuracy: The analyte standard of this ELISA was calibrated against NIBSC* international standard 93/722.
• Recovery: A mid-curve recovery of 76 - 86% was determined by spiking defined amounts of analyte standard into serum or plasma samples in repeated experiments.
• Precision: The intra-assay precision of this assay is 2.5% (CV). The inter-assay precision of this assay is 9.8% (CV).
*National Institute of Biological Standards and Control, Potters Bar, Hertfordshire EN6 3QG, UK.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
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Human IL-10 ELISA Kit
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