Human Bone Marrow Stromal Cells, Frozen

Primary human cells, frozen

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Human Bone Marrow Stromal Cells, Frozen

Primary human cells, frozen

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Primary human cells, frozen
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Human primary stromal cells (mesenchymal stem and progenitor cells) are produced by expanding bone marrow mononuclear cells (MNCs) in culture and cryopreserved following the first passage in culture. Human Bone Marrow Stromal Cells Derived in ACF Medium, Frozen were cultured in MesenCult™-ACF Plus Culture Kit (Catalog #05448).071) were cultured in MesenCult™-ACF Plus Culture Kit (Catalog #05448).

Cells were obtained using Institutional Review Board (IRB)-approved consent forms and protocols.

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Browse our Frequently Asked Questions (FAQs) on Primary Cells.
• CryoStor® CS10
Cell Type
Mesenchymal Stem and Progenitor Cells
Cell and Tissue Source
Bone Marrow
≥ 90% CD73+, ≥ 90% CD90+, ≥ 90% CD105+, ≤ 5% CD14+, ≤ 5% CD34+, and ≤ 5% CD45+ by flow cytometry.

Data Figures

Figure 1. Human Bone Marrow Stromal Cells Cultured Using the MesenCult™-ACF Plus Culture Kit Maintain Multi-Lineage Differentiation Potential

Human bone marrow stromal cells (Catalog #70071) derived and expanded using the MesenCult™-ACF Plus Culture Kit (Catalog #05448) differentiate to A) adipocytes (Oil Red O staining), B) chondrocytes (Alcian Blue and Nuclear Fast Red staining), and C) osteoblasts (Alizarin Red S staining).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

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This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications

Publications (4)

Reprogramming Postnatal Human Epidermal Keratinocytes Toward Functional Neural Crest Fates. Bajpai VK et al. Stem cells (Dayton, Ohio) 2017 JAN


During development, neural crest (NC) cells are induced by signaling events at the neural plate border of all vertebrate embryos. Initially arising within the central nervous system, NC cells subsequently undergo an epithelial to mesenchymal transition to migrate into the periphery, where they differentiate into diverse cell types. Here we provide evidence that postnatal human epidermal keratinocytes (KC), in response to fibroblast growth factor 2 and insulin like growth factor 1 signals, can be reprogrammed toward a NC fate. Genome-wide transcriptome analyses show that keratinocyte-derived NC cells are similar to those derived from human embryonic stem cells. Moreover, they give rise in vitro and in vivo to NC derivatives such as peripheral neurons, melanocytes, Schwann cells and mesenchymal cells (osteocytes, chondrocytes, adipocytes, and smooth muscle cells). By demonstrating that human keratin-14+ KC can form NC cells, even from clones of single cells, our results have important implications in stem cell biology and regenerative medicine. Stem Cells 2017.
Mesenchymal stem cells cultured on magnetic nanowire substrates. Perez JE et al. Nanotechnology 2017 FEB


Stem cells have been shown to respond to extracellular mechanical stimuli by regulating their fate through the activation of specific signaling pathways. In this work, an array of iron nanowires (NWs) aligned perpendicularly to the surface was fabricated by pulsed electrodepositon in porous alumina templates followed by a partial removal of the alumina to reveal 2-3 μm of the NWs. This resulted in alumina substrates with densely arranged NWs of 33 nm in diameter separated by 100 nm. The substrates were characterized by scanning electron microscopy (SEM) energy dispersive x-ray analysis and vibrating sample magnetometer. The NW array was then used as a platform for the culture of human mesenchymal stem cells (hMSCs). The cells were stained for the cell nucleus and actin filaments, as well as immuno-stained for the focal adhesion protein vinculin, and then observed by fluorescence microscopy in order to characterize their spreading behavior. Calcein AM/ethidium homodimer-1 staining allowed the determination of cell viability. The interface between the cells and the NWs was studied using SEM. Results showed that hMSCs underwent a re-organization of actin filaments that translated into a change from an elongated to a spherical cell shape. Actin filaments and vinculin accumulated in bundles, suggesting the attachment and formation of focal adhesion points of the cells on the NWs. Though the overall number of cells attached on the NWs was lower compared to the control, the attached cells maintained a high viability (>90%) for up to 6 d. Analysis of the interface between the NWs and the cells confirmed the re-organization of F-actin and revealed the adhesion points of the cells on the NWs. Additionally, a net of filopodia surrounded each cell, suggesting the probing of the array to find additional adhesion points. The cells maintained their round shape for up to 6 d of culture. Overall, the NW array is a promising nanostructured platform for studying and influencing hMSCs differentiation.
Exploring continuous and integrated strategies for the up- and downstream processing of human mesenchymal stem cells. Cunha B et al. Journal of biotechnology 2015 NOV


The integration of up- and downstream unit operations can result in the elimination of hold steps, thus decreasing the footprint, and ultimately can create robust closed system operations. This type of design is desirable for the bioprocess of human mesenchymal stem cells (hMSC), where high numbers of pure cells, at low volumes, need to be delivered for therapy applications. This study reports a proof of concept of the integration of a continuous perfusion culture in bioreactors with a tangential flow filtration (TFF) system for the concentration and washing of hMSC. Moreover, we have also explored a continuous alternative for concentrating hMSC. Results show that expanding cells in a continuous perfusion operation mode provided a higher expansion ratio, and led to a shift in cells' metabolism. TFF operated either in continuous or discontinuous allowed to concentrate cells, with high cell recovery (>80%) and viability (>95%); furthermore, continuous TFF permitted to operate longer with higher cell concentrations. Continuous diafiltration led to higher protein clearance (98%) with lower cell death, when comparing to discontinuous diafiltration. Overall, an integrated process allowed for a shorter process time, recovering 70% of viable hMSC (>95%), with no changes in terms of morphology, immunophenotype, proliferation capacity and multipotent differentiation potential.
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