EasySep™ Human ILC2 Enrichment Kit

Immunomagnetic negative selection cell enrichment kit
EasySep™ Human ILC2 Enrichment Kit

Immunomagnetic enrichment of untouched human ILC2 cells

1 x 10^9 cells
Catalog # 17972
729 USD
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Required Products
  1. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

  2. "The Big Easy" EasySep™ Magnet
    "The Big Easy" EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

Overview

The EasySep™ Human ILC2 enrichment kit is designed to enrich Group 2 Innate Lymphoid Cells (ILC2s) from leukapheresis products by immunomagnetic negative selection. Unwanted cells are targeted for removal by Tetrameric Antibody Complexes directed against non-ILC2s and dextran-coated magnetic particles (RapidSpheres™). Labeled cells are separated using an EasySep™ magnet without the use of columns. Unwanted cells remain in the tube as desired cells are simply poured off into a new tube. Isolated cells are immediately available for downstream applications such as flow cytometry and cell sorting.
Advantages
• Fast, easy-to-use and column-free
• Untouched, viable cells
• Facilitates rapid flow sorting of ILC2s
Components
  • EasySep™ Human ILC2 Enrichment Kit (Catalog #17972)
    • EasySep™ Human ILC2 Enrichment Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50103, 1 mL
Magnet Compatibility
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
 
Subtype
Cell Isolation Kits
Cell Type
Innate Lymphoid Cells
Species
Human
Sample Source
Leukapheresis
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep
Area of Interest
Immunology

Related Products

Labeling Antibodies

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
EasySep™ Human ILC2 Enrichment Kit
Catalog #
17972
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human ILC2 Enrichment Kit
Catalog #
17972
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human ILC2 Enrichment Kit
Catalog #
17972
Lot #
All
Language
English

Educational Materials(4)

Brochure
Cell Enrichment Prior to Cell Sorting
Brochure
EasySep™ Cell Separation Technology
Technical Bulletin
Gating Strategy for the Identification of ILC2s
Wallchart
Human Immune Cytokines

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data


Figure 1. Typical EasySep™ Human ILC2 Enrichment Profile

Starting with washed leukapheresis sample, the ILC2 content (Lin-CD45+CD294+CD127+CD161+) of the enriched fraction typically ranges from 13 - 78%. In the above example, the percentages of ILC2s in the start and final enriched fractions are 0.03% and 35.3%, respectively. NOTE: The ILC2 content of the start fraction typically ranges from 0.001 - 0.16%.

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