EasySep™ Human ILC2 Enrichment Kit

Immunomagnetic negative selection cell enrichment kit

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EasySep™ Human ILC2 Enrichment Kit

Immunomagnetic enrichment of untouched human ILC2 cells

1 x 109 cells
Catalog #17972
729 USD

Required Products

Overview Related Products Scientific Resources Product Applications Data and Publications

Overview

The EasySep™ Human ILC2 enrichment kit is designed to enrich Group 2 Innate Lymphoid Cells (ILC2s) from leukapheresis products by immunomagnetic negative selection. Unwanted cells are targeted for removal by Tetrameric Antibody Complexes directed against non-ILC2s and dextran-coated magnetic particles (RapidSpheres™). Labeled cells are separated using an EasySep™ magnet without the use of columns. Unwanted cells remain in the tube as desired cells are simply poured off into a new tube. Isolated cells are immediately available for downstream applications such as flow cytometry and cell sorting.
Advantages:
• Fast, easy-to-use and column-free
• Untouched, viable cells
• Facilitates rapid flow sorting of ILC2s
Components:
  • EasySep™ Human ILC2 Enrichment Kit (Catalog #17972)
    • EasySep™ Human ILC2 Enrichment Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50103, 1 mL
Magnet Compatibility:
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
 
Subtype:
Cell Isolation Kits
Cell Type:
Innate Lymphoid Cells
Species:
Human
Sample Source:
Leukapheresis; PBMC
Selection Method:
Negative
Application:
Cell Isolation
Brand:
EasySep
Area of Interest:
Immunology

Scientific Resources

Product Documentation

Educational Materials

(5)
Brochure
EasySep™ Cell Separation Technology
Brochure
Cell Enrichment Prior to Cell Sorting
Technical Bulletin
Gating Strategy for the Identification of ILC2s
Wallchart
Human Immune Cytokines
Scientific Poster
Rapid Isolation of Functional Human ILC2 from Whole Blood and Leuko Pak

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area Workflow Stages for
Workflow Stages
Innate Lymphoid Cell Research
Cell Sourcing
Cell Isolation
Cell Culture and Characterization

Data and Publications

Data


Figure 1. Typical EasySep™ Human ILC2 Enrichment Profile

Starting with fresh PBMCs, the ILC2 content (Lin-CD45+CD294+CD127+CD161+) of the enriched fraction typically ranges from 13 - 78%. In the above example, the percentages of ILC2s in the start and final enriched fractions are 0.03% and 35.3%, respectively. NOTE: The ILC2 content of the start fraction typically ranges from 0.001 - 0.16%.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.