EasySep™ Mouse CD138 Positive Selection Kit

Immunomagnetic positive selection kit

New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

EasySep™ Mouse CD138 Positive Selection Kit

Immunomagnetic positive selection kit

From: 726 USD
Catalog #
18957_C
Immunomagnetic positive selection kit

Product Advantages


  • Fast, easy-to-use and column-free

  • Up to 97% purity with immunized mice

  • Useful for the enrichment of plasma cells

  • Compatible with hybridoma generation protocols, including electrofusion

What's Included

  • EasySep™ Mouse CD138 Positive Selection Kit (Catalog #18957)
    • EasySep™ Mouse CD138 Positive Selection Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 1 mL
    • EasySep™ Mouse FcR Blocker, 2 x 0.5 mL
  • RoboSep™ Mouse CD138 Positive Selection Kit (Catalog #18957RF)
    • EasySep™ Mouse CD138 Positive Selection Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 1 mL
    • EasySep™ Mouse FcR Blocker, 2 x 0.5 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
    • EasySep™ EasyTube™-14 (Catalog #20128) x 2
New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

Overview

The EasySep™ Mouse CD138 Positive Selection Kit is designed to isolate CD138+ cells from mouse splenocytes, lymph nodes and bone marrow by immunomagnetic positive selection. Desired cells are targeted with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Isolated cells are immediately available for downstream applications such as flow cytometry, cell culture, and hybridoma generation.
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
B Cells, Plasma
Species
Mouse
Sample Source
Bone Marrow, Lymph Node, Other, Spleen
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology

Data Figures

Figure 1. Typical EasySep™ Mouse CD138 Positive Selection Kit Isolation Profile

(A) Starting with immunized BALB/c mouse splenocytes, the CD138+ cell content of the isolated fraction is typically 81.5 ± 4.9% (mean ± SD). In the above example, the purities of the start and final isolated fractions are 4.6% and 83.3%, respectively. (B) Starting with immunized BALB/c mouse splenocytes, the plasma cell (CD138+CD267 (TACI)+) content is typically 68.5 ± 11.3% (mean ± SD). In the above example, the purities of the start and final isolated fractions are 1.3% and 70.2%, respectively. (C) Starting with naïve C57BL/6 mouse splenocytes, the CD138+ cell content of the isolated fraction is typically 78.3 ± 5.7% (mean ± SD). In the above example, the purities of the start and final isolated fractions are 5.0% and 79.5%, respectively. (D) Starting with naïve C57BL/6 mouse splenocytes, the plasma cell (CD138+CD267 (TACI)+) content is typically 50.8 ± 10.0 % (mean ± SD). In the above example, the purities of the start and final isolated fractions are 0.4% and 56.3%, respectively. (E) Isolated CD138+ cells or total splenocytes from mice immunized with various antigens were fused with Sp2/0 mouse myeloma cells and plated in semi-solid medium using ClonaCell™-HY Hybridoma Kit (Catalog #03800). The % antigen-specific hit rate was determined by ELISA. The % antigen-specific hit rates for total splenocytes and CD138+ cells were 4.1 ± 1.6% and 28.8 ± 11.0% (mean ± SD), respectively. (F) Isolated CD138+ cells or total splenocytes from mice immunized with various antigens were fused with Sp2/0 mouse myeloma cells and plated in semi-solid medium using ClonaCell™-HY Hybridoma Kit (Catalog #03800). The % antibody-secreting hybridomas was determined by ELISA. The % antibody-secreting hybridomas for total splenocytes and CD138+ cells were 33.0 ± 8.7% and 99.3 ± 0.6% (mean ± SD), respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
18957
Lot #
All
Language
English
Catalog #
18957RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
18957
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
18957
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
18957
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
18957RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
18957RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
18957RF
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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