EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II

Immunomagnetic positive selection cell isolation kit

New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II

Immunomagnetic positive selection cell isolation kit

From: 752 USD
Catalog #
17887_C
Immunomagnetic positive selection cell isolation kit

Product Advantages


  • Fast and easy-to-use

  • No columns required

What's Included

  • EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II (Catalog #17887)
    • EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Cocktail, 3 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 3 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
  • RoboSep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II (Catalog #17887RF)
    • EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Cocktail, 3 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 3 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
    • RoboSep™ Buffer (Catalog #20104) x 2
    • RoboSep™ Filter Tips (Catalog #20125) x 2
New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
Products for Your Protocol

Overview

The EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit is designed to isolate CD138+ (syndecan-1) cells from whole blood and bone marrow by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD138 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD138 antigen is expressed on normal and malignant plasma cells (but not mature B cells).

This product replaces the EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit (Catalog #18387) for even faster cell isolations.
Magnet Compatibility
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
Myeloma, Plasma
Species
Human
Sample Source
Bone Marrow, Whole Blood
Selection Method
Positive
Application
ELISA
Brand
EasySep, RoboSep
Area of Interest
Immunology

Data Figures

Starting with fresh whole blood spiked with a multiple myeloma cell line, U266, the CD138+ cell content of the selected fraction typically ranges from 83.7 - 98.3%. In the above example, the purities of the start and final isolated fractions are 9.1% and 90.4%, respectively. NOTE: For samples with CD138+ starting frequency < 10 - 15%, the CD138+ purity of the isolated fraction may be variable. NOTE: Red blood cells were removed from the start sample by lysis prior to flow cytometry.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
17887
Lot #
Lot 1000052960 and below
Language
English
Catalog #
17887
Lot #
Lot 1000058250 and above
Language
English
Catalog #
17887
Lot #
Lot 1000058250 and above
Language
Multi
Catalog #
17887RF
Lot #
Lot 1000052960 and below
Language
English
Catalog #
17887RF
Lot #
Lot 1000058250 and above
Language
English
Catalog #
17887RF
Lot #
Lot 1000058250 and above
Language
Multi
Document Type
Safety Data Sheet 1
Catalog #
17887
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
17887
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
17887
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
17887RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
17887RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
17887RF
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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