Anti-Human CD138 (Syndecan-1) Antibody, Clone MI15
Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD138 (syndecan-1)
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Overview
Data Figures
Figure 1. Data for APC-Conjugated
(A) Flow cytometry analysis of human U266 myeloma cells labeled with Anti-Human CD138 (Syndecan-1) Antibody, Clone MI15, APC (filled histogram) or a mouse IgG1, kappa APC isotype control antibody (solid line histogram). (B) Flow cytometry analysis of human U266 myeloma cells isolated with EasySep™ Human CD138 Positive Selection Kit from a mixed population of U266 cells and human peripheral blood mononuclear cells. Cells were labeled with Anti-Human CD138 (Syndecan-1) Antibody, Clone MI15, APC. Histograms show labeling of the starting population containing ~5% U266 cells (Start) and the isolated cells (Isolated). Labeling of the start cells with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, APC (Catalog #60071AZ) is shown (solid line histogram).
Figure 2. Data for FITC-Conjugated
(A) Flow cytometry analysis of human U266 myeloma cells labeled with Anti-Human CD138 (Syndecan-1) Antibody, Clone MI15, FITC (filled histogram) or a mouse IgG1, kappa FITC isotype control antibody (solid line histogram). (B) Flow cytometry analysis of human U266 myeloma cells isolated with EasySep™ Human CD138 Positive Selection Kit from a mixed population of U266 cells and human peripheral blood mononuclear cells. Cells were labeled with Anti-Human CD138 (Syndecan-1) Antibody, Clone MI15, FITC. Histograms show labeling of the starting population containing ~5% U266 cells (Start) and the isolated cells (Isolated). Labeling of the start cells with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, FITC (Catalog #60071FI) is shown (solid line histogram).
Figure 3. Data for PE-Conjugated
(A) Flow cytometry analysis of human U266 myeloma cells labeled with Anti-Human CD138 (Syndecan-1) Antibody, Clone MI15, PE (filled histogram) or a mouse IgG1, kappa PE isotype control antibody (solid line histogram). (B) Flow cytometry analysis of human U266 myeloma cells isolated with EasySep™ Human CD138 Positive Selection Kit from a mixed population of U266 cells and human peripheral blood mononuclear cells. Cells were labeled with Anti-Human CD138 (Syndecan-1) Antibody, Clone MI15, PE. Histograms show labeling of the starting population containing ~5% U266 cells (Start) and the isolated cells (Isolated). Labeling of the start cells with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PE (Catalog #60071PE) is shown (solid line histogram).
Figure 4. Data for Unconjugated
Flow cytometry analysis of human U266 myeloma cells labeled with Anti-Human CD138 (Syndecan-1) Antibody, Clone MI15, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI; filled histogram) or a mouse IgG1, kappa isotype control antibody followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (open histogram).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (3)
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Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
