EasySep™ Human Pan-Extracellular Vesicle Positive Selection Kit
Fast & easy isolation of human extracellular vesicles using immunomagnetic positive selection
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EasySep™ Human Pan-Extracellular Vesicle Positive Selection Kit -
Label for EasySep™ Human Pan-Extracellular Vesicle Positive Selection Kit
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Required Products
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Magnet for column-free immunomagnetic separation -
Dulbecco’s phosphate-buffered saline without calcium and magnesium
Overview
The EasySep™ Human Pan-Extracellular Vesicle Positive Selection Kit isolates highly purified human extracellular vesicles from plasma/serum and cell culture conditioned medium by immunomagnetic positive selection. Extracellular vesicles are labeled with Tetrameric Antibody Complexes recognizing CD9, CD81, and CD63, and magnetic particles. Labeled extracellular vesicles are separated without columns using an EasySep™ magnet. Unwanted biofluid components are simply poured off, while desired extracellular vesicles remain in the tube. Isolated extracellular vesicles are available for downstream applications such as DNA/RNA extraction, Western blot, or mass spectometry.
Advantages:
⦁ Fast, easy to use, and column-free.
⦁ Avoid the need for ultracentrifugation and associated time consuming EV isolation methods.
⦁ Obtain highly purified EVs without contaminating biofluid components.
⦁ Avoid the need for ultracentrifugation and associated time consuming EV isolation methods.
⦁ Obtain highly purified EVs without contaminating biofluid components.
Components:
- EasySep™ Human Pan-Extracellular Vesicle Positive Selection Kit (Catalog #17891)
- EasySep™ Human Pan-Extracellular Vesicle Positive Selection Cocktail 1 x 1 mL
- EasySep™ Releasable RapidSpheres™ 50201, 2 x 1 mL
- NOTE: Isolated extracellular vesicles should not be released from the EasySep™ Releasable Rapidspheres™ 50201
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
Subtype:
Cell Isolation Kits
Cell Type:
Other
Sample Source:
Other
Selection Method:
Positive
Application:
Cell Isolation
Brand:
EasySep
Area of Interest:
Extracellular Vesicle Research
Related Products
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Falcon® Round-Bottom Polystyrene Tubes, 5 mL
Sterile polystyrene round-bottom tube for cell separation and cell culture; with or without caps
Scientific Resources
Product Documentation
Document Type
Product Name
Catalog #
Lot #
Language
17891
All
17891
All
Educational Materials
(3)
Data and Publications
Data
Figure 1. Typical Western Blot Analyses of EVs Isolated from Human Plasma and Mesenchymal Stromal Cell (MSC)-Conditioned Medium
The Western blot analyses in the above examples show positive isolation of EVs with CD9, CD63, and CD81 tetraspanin markers from (A) human plasma and (B) MSC-conditioned medium. Western blots were run under non-reducing conditions.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.